PMID- 12270665
OWN - NLM
STAT- MEDLINE
DCOM- 20021204
LR  - 20190901
IS  - 0166-0934 (Print)
IS  - 0166-0934 (Linking)
VI  - 105
IP  - 2
DP  - 2002 Sep
TI  - Characterisation of a human herpesvirus 6 variant A 'amplicon' and replication 
      modulation by U94-Rep 'latency gene'.
PG  - 331-41
AB  - The human herpesvirus 6 (HHV-6) variant A genome has conserved sequences which 
      are signals for initiating lytic replication (origin, 'ori-lyt') and DNA 
      packaging into the virion (pac2/1). Here these are functionally characterised and 
      used to construct a gene-expression amplifiable-vector, an 'amplicon', with 
      applications for gene delivery to lymphoid-myeloid cells or their progenitor stem 
      cells. A minimal efficient ori-lyt for replication was identified which was 
      enhanced in the presence of the imperfect direct repeated DNA domain (IDR). In A 
      variant strains these are arranged as three adjacent repeats with the most 
      divergence in IDR3. Addition of the pac2/1 sequences also enhanced detection of 
      ori-lyt replication and conferred DNA packaging properties, thus, the amplicon 
      could be packaged with 'helper' virus. An HHV-6 specific factor, which inhibits 
      amplicon replication was identified by trans replication assays. This is the 
      U94-Rep 'latency' gene product, which can modulate efficiency of such amplifiable 
      vectors, based on the lytic origin. It could also affect maintenance of viral 
      genomes or vectors during latency.
FAU - Turner, Simon
AU  - Turner S
AD  - Department of Infectious and Tropical Diseases, London School of Hygiene and 
      Tropical Medicine, University of London, Keppel St., London WC1E 7HT, UK.
FAU - DiLuca, Dario
AU  - DiLuca D
FAU - Gompels, Ursula
AU  - Gompels U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (DNA Primers)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - Gene Amplification
MH  - *Genetic Variation
MH  - Herpesvirus 6, Human/*classification/*genetics/isolation & purification
MH  - Humans
MH  - Jurkat Cells
MH  - Plasmids
MH  - Polymerase Chain Reaction/methods
MH  - Restriction Mapping/methods
MH  - T-Lymphocytes/virology
MH  - Virus Replication/*physiology
EDAT- 2002/09/25 06:00
MHDA- 2002/12/05 04:00
CRDT- 2002/09/25 06:00
PHST- 2002/09/25 06:00 [pubmed]
PHST- 2002/12/05 04:00 [medline]
PHST- 2002/09/25 06:00 [entrez]
AID - S0166093402001301 [pii]
AID - 10.1016/s0166-0934(02)00130-1 [doi]
PST - ppublish
SO  - J Virol Methods. 2002 Sep;105(2):331-41. doi: 10.1016/s0166-0934(02)00130-1.