PMID- 1234056 OWN - NLM STAT- MEDLINE DCOM- 19770103 LR - 20181130 IS - 0014-2980 (Print) IS - 0014-2980 (Linking) VI - 5 IP - 3 DP - 1975 Mar TI - Inactivation of hapten-modified bacteriophage by antibody: increased sensitivity of the assay in media containing polyethylene glycol. PG - 216-8 AB - Inactivation of dinitrophenylated bacteriophage T4 by porcine and rabbit anti-dinitrophenyl (DNP) antibodies was studied by the complex inactivation methods. The inactivation titers increased 20-30 times when polyethylene glycol, mol. wt. 6000, was added to the incubation media. Consequently, the detection of dinitrophenylated serum albumin with the modified bacteriophage could be carried out with higher sensitivity upon addition of polyethylene glycol. FAU - Franek, F AU - Franek F FAU - Doskocil, J AU - Doskocil J LA - eng PT - Journal Article PL - Germany TA - Eur J Immunol JT - European journal of immunology JID - 1273201 RN - 0 (Antibodies) RN - 0 (Antigens) RN - 0 (Culture Media) RN - 0 (Dinitrobenzenes) RN - 0 (Haptens) RN - 3WJQ0SDW1A (Polyethylene Glycols) SB - IM MH - Antibodies/*analysis MH - Antigens/*analysis MH - Coliphages MH - Culture Media MH - Dinitrobenzenes/immunology MH - Haptens MH - Methods MH - *Polyethylene Glycols EDAT- 1975/03/01 00:00 MHDA- 1975/03/01 00:01 CRDT- 1975/03/01 00:00 PHST- 1975/03/01 00:00 [pubmed] PHST- 1975/03/01 00:01 [medline] PHST- 1975/03/01 00:00 [entrez] AID - 10.1002/eji.1830050313 [doi] PST - ppublish SO - Eur J Immunol. 1975 Mar;5(3):216-8. doi: 10.1002/eji.1830050313.