PMID- 12351396
OWN - NLM
STAT- MEDLINE
DCOM- 20021205
LR  - 20210206
IS  - 0006-4971 (Print)
IS  - 0006-4971 (Linking)
VI  - 100
IP  - 8
DP  - 2002 Oct 15
TI  - Myeloid blood CD11c(+) dendritic cells and monocyte-derived dendritic cells 
      differ in their ability to stimulate T lymphocytes.
PG  - 2858-66
AB  - Dendritic cells (DCs) initiate and direct immune responses. Recent studies have 
      defined different DC populations, therefore we undertook this study comparing 2 
      types of myeloid DCs: blood CD11c(+) DCs and in vitro monocyte-derived DCs 
      (Mo-DCs), which are both candidates as cellular adjuvants for cancer 
      immunotherapy. Blood CD11c(+) DCs were prepared by cell sorting from peripheral 
      blood mononuclear cells cultured overnight in RPMI 1640 medium supplemented with 
      autologous or pooled AB serum. Mo-DCs were prepared in the same medium using 
      granulocyte macrophage-colony-stimulating factor (GM-CSF)/interleukin 4 (IL-4) 
      and differentiated/activated with lipopolysaccharide or monocyte-conditioned 
      medium (ActMo-DCs). Morphologically, differences between the DC preparations were 
      noted both at a light and and electron microscopic level. Blood CD11c(+) DCs 
      expressed similar levels of HLA-DR, CD40, CD86, and CD83 as Mo-DCs. CD209 was 
      present on Mo-DCs but not on blood CD11c(+) DCs. Blood CD11c(+) DCs generated a 
      lower proliferative mixed leukocyte response (MLR) than Mo-DCs. Blood CD11c(+) 
      DCs loaded with 0.1 microg/mL tetanus toxoid (TT)-generated greater T lymphocyte 
      proliferative responses than did Mo-DCs or ActMo-DCs, but when loaded with higher 
      TT concentrations no difference in T lymphocyte proliferative response was 
      observed. Keyhole limpet hemocyanin (KLH)-loaded blood CD11c(+) DCs generated 
      greater T lymphocyte proliferative responses than Mo-DCs or ActMo-DCs. Allogeneic 
      MLR- or KLH-specific responses induced by blood CD11c(+) DCs generated more Th1 
      effectors than the responses induced by Mo-DCs or ActMo-DCs. These data establish 
      several differences in the properties of blood CD11c(+) DCs, Mo-DCs, and 
      ActMo-DCs, which suggest that blood DCs merit further consideration as DC 
      preparations for clinical programs are evolved.
FAU - Osugi, Yuko
AU  - Osugi Y
AD  - Department of Developmental Medicine (Pediatrics), D-5, Osaka University Graduate 
      School of Medicine, Japan.
FAU - Vuckovic, Slavica
AU  - Vuckovic S
FAU - Hart, Derek N J
AU  - Hart DN
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Blood
JT  - Blood
JID - 7603509
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD11c Antigen)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - CD11c Antigen/*blood
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*immunology
MH  - Humans
MH  - Interferon-gamma/blood
MH  - Lymphocyte Activation/*immunology
MH  - Monocytes/cytology/*immunology
MH  - T-Lymphocytes/cytology/*immunology
EDAT- 2002/09/28 04:00
MHDA- 2002/12/06 04:00
CRDT- 2002/09/28 04:00
PHST- 2002/09/28 04:00 [pubmed]
PHST- 2002/12/06 04:00 [medline]
PHST- 2002/09/28 04:00 [entrez]
AID - S0006-4971(20)50948-8 [pii]
AID - 10.1182/blood.V100.8.2858 [doi]
PST - ppublish
SO  - Blood. 2002 Oct 15;100(8):2858-66. doi: 10.1182/blood.V100.8.2858.