PMID- 12352395
OWN - NLM
STAT- MEDLINE
DCOM- 20021018
LR  - 20230302
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 168
IP  - 4 Pt 1
DP  - 2002 Oct
TI  - Analysis of numerical chromosomal aberrations in adrenal cortical neoplasms by 
      fluorescence in situ hybridization.
PG  - 1370-3
AB  - PURPOSE: We identified numerical chromosomal aberrations in adrenal cortical 
      neoplasms using interphase fluorescence in situ hybridization (FISH) and 
      correlated these aberrations with DNA ploidy and endocrine dysfunction. MATERIALS 
      AND METHODS: Our study included 25 adenomas and 2 carcinomas associated with 
      primary aldosteronism or Cushing's syndrome. Eight normal adrenal tissue samples 
      served as controls. Isolated nuclei from frozen samples were used for FISH and 
      formalin fixed, paraffin embedded tissues from the same materials were analyzed 
      by flow cytometry for DNA ploidy. For FISH we used centromere specific probes for 
      chromosomes 3, 7, 8, 11 and 12. RESULTS: None of the normal adrenal tissues had 
      any numerical chromosomal aberrations in any chromosome analyzed or any abnormal 
      findings on DNA ploidy analysis. Tetrasomy of chromosomes 3, 7, 8, 11 and 12 was 
      detected in 8, 13, 14, 11 and 12 of the 17 adenomas associated with primary 
      aldosteronism, and in 2, 0, 0, 0 and 0 of the 8 associated with Cushing's 
      syndrome, respectively. DNA flow cytometry revealed tetraploidy in 11 of the 17 
      cases of primary aldosteronism and in 1 of the 8 of Cushing's syndrome. Five 
      diploid adenomas associated with primary aldosteronism also showed tetrasomy in 2 
      or more chromosomes. One of the 2 carcinomas showed aneuploidy and aneusomy of 
      chromosomes 8, 11 and 12 but the other showed no abnormal peaks on DNA 
      histography and no numerical chromosomal aberrations. CONCLUSIONS: All 
      chromosomes analyzed in adenomas associated with primary aldosteronism frequently 
      showed tetrasomy, whereas few chromosomal abnormalities were detected in adenomas 
      associated with Cushing's syndrome. Our results indicate that DNA tetraploidy is 
      common in adrenal cortical adenomas associated with primary aldosteronism. 
      Interphase FISH strongly supported flow cytometry findings and could provide 
      further information on individual chromosomes.
FAU - Shono, Takefumi
AU  - Shono T
AD  - Department of Urology, Nagasaki University School of Medicine, Sakamoto, Japan.
FAU - Sakai, Hideki
AU  - Sakai H
FAU - Takehara, Kousuke
AU  - Takehara K
FAU - Honda, Sumihisa
AU  - Honda S
FAU - Kanetake, Hiroshi
AU  - Kanetake H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adrenal Cortex/pathology
MH  - Adrenocortical Adenoma/*genetics/pathology
MH  - Adrenocortical Carcinoma/*genetics/pathology
MH  - Adult
MH  - Carcinoma/genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosome Mapping
MH  - Cushing Syndrome/genetics/pathology
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Hyperaldosteronism/genetics/pathology
MH  - *In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Paraneoplastic Endocrine Syndromes/*genetics/pathology
MH  - Ploidies
EDAT- 2002/09/28 04:00
MHDA- 2002/10/19 04:00
CRDT- 2002/09/28 04:00
PHST- 2002/09/28 04:00 [pubmed]
PHST- 2002/10/19 04:00 [medline]
PHST- 2002/09/28 04:00 [entrez]
AID - S0022-5347(05)64451-5 [pii]
AID - 10.1016/S0022-5347(05)64451-5 [doi]
PST - ppublish
SO  - J Urol. 2002 Oct;168(4 Pt 1):1370-3. doi: 10.1016/S0022-5347(05)64451-5.