PMID- 12352915
OWN - NLM
STAT- MEDLINE
DCOM- 20021010
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 74
IP  - 4
DP  - 2002 Aug 27
TI  - Flow cytometric evaluation of pregnancy-induced anti-HLA immunization and blood 
      transfusion-induced reactivation.
PG  - 537-40
AB  - BACKGROUND: Pregnancy-induced alloimmunization (PIA) may decrease to a level that 
      becomes undetectable by complement-dependent cytotoxicity (CDC). Nevertheless, 
      such alloimmunization may provoke acute rejections after kidney transplantation 
      and lead to broad-spectrum immunizations after transfusion. Flow-cytometry (FC) 
      was used to estimate the frequency of low-level PIA and to evaluate its influence 
      on posttransfusion alloimmunization profiles. METHODS: To evaluate the frequency 
      of low-level PIA, the sera of 36 women, free of CDC-detectable anti-HLA IgG 
      (CDC-IgG- negative), were cross-matched by FC against their husband's or 
      offspring's lymphocytes and further analyzed with human leukocyte antigen (HLA) 
      Ag-coated microbeads (Flow-PRA One-Lambda, Canoga Park, CA). To evaluate the 
      influence of low-level alloimmunization on posttransfusion appearance of CDC-IgG, 
      pretransfusion sera of a second cohort of 43 women, also CDC-IgG-negative and 
      included in a transfusion protocol, were analyzed by Flow-PRA. Posttransfusion 
      sera were analyzed for the development of cytotoxic IgG. RESULTS: Ten of the 
      first cohort of 36 (27.8%) CDC-IgG-negative women showed a positive FC 
      cross-match against the husband or offspring lymphocytes. Flow-PRA analysis 
      confirmed that 9 of 10 positive cross-matched sera contained anti-HLA IgG. Among 
      the 43 transfused patients, 11 of 16 (68.7%) of the women who were 
      CDC-IgG-positive after blood transfusion showed FC-detectable IgG before 
      transfusion; although 2 of 27 (7.4%) of the patients who remained 
      CDC-IgG-negative after transfusion showed FC-detectable IgG before transfusion (P 
      <0.001). CONCLUSION: Most of the de novo anti-HLA immunizations detected by CDC 
      after transfusion in previously pregnant women can be detected by Flow-PRA before 
      transfusion.
FAU - Rebibou, Jean-Michel
AU  - Rebibou JM
AD  - INSERM E0119-UPRES EA2284 Laboratoire d'immunogenetique EFS de 
      Bourgogne/Franche-Comte, Besancon, France.
FAU - Chabod, Jacqueline
AU  - Chabod J
FAU - Alcalay, Dominique
AU  - Alcalay D
FAU - Coussediere, Marie-Christine
AU  - Coussediere MC
FAU - Deteix, Patrice
AU  - Deteix P
FAU - Touchard, Guy
AU  - Touchard G
FAU - Dupont, Isabelle
AU  - Dupont I
FAU - Thevenin, Chantal
AU  - Thevenin C
FAU - Chalopin, Jean-Marc
AU  - Chalopin JM
FAU - Tiberghien, Pierre
AU  - Tiberghien P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - *Blood Transfusion
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Histocompatibility Antigens Class I/*immunology
MH  - Histocompatibility Antigens Class II/*immunology
MH  - Histocompatibility Testing
MH  - Humans
MH  - Immunity/physiology
MH  - Immunoglobulin G/blood
MH  - Male
MH  - Pregnancy/*immunology
EDAT- 2002/09/28 04:00
MHDA- 2002/10/11 04:00
CRDT- 2002/09/28 04:00
PHST- 2002/09/28 04:00 [pubmed]
PHST- 2002/10/11 04:00 [medline]
PHST- 2002/09/28 04:00 [entrez]
AID - 10.1097/00007890-200208270-00018 [doi]
PST - ppublish
SO  - Transplantation. 2002 Aug 27;74(4):537-40. doi: 10.1097/00007890-200208270-00018.