PMID- 12353141
OWN - NLM
STAT- MEDLINE
DCOM- 20030114
LR  - 20061115
IS  - 0944-1166 (Print)
IS  - 0944-1166 (Linking)
VI  - 9
IP  - 3
DP  - 2002
TI  - Comparative genomic hybridization (CGH) and fluorescence in situ hybridization 
      (FISH) in the diagnosis of hepatocellular carcinoma.
PG  - 304-11
AB  - BACKGROUND: The histomorphological diagnosis of well differentiated 
      hepatocellular carcinoma (HCC) may be a challenging task, because regenerative 
      nodules and adenomas share similar microscopic features. Moreover, the 
      differentiation of intrahepatic metastatic spread from multicentric growth in 
      multinodular HCC is frequently not possible by histological criteria alone. 
      Whether molecular cytogenetic analysis can contribute to the differential 
      diagnosis of HCC was recently investigated by our group. METHODS: We applied 
      comparative genomic hybridization (CGH) and fluorescence in situ hybridization 
      (FISH) to 12 cases of hepatocellular adenoma (HCA) and 28 cases of well 
      differentiated HCC. RESULTS: CGH revealed aberrations in 2/6 HCAs, affecting 7p 
      in 1 sample each and 17q and 20 in the second case. In 4/4 well differentiated 
      HCCs, aberrations occurred, with a mean of eight aberrations per case, focused on 
      1q, 4q, 8p, 8q, 16p, and 17p. In all HCC samples, at least two of these sites 
      were affected. In 2 multinodular HCCs, six and four different tumor nodes, 
      respectively, were analyzed. In the first case, aberration patterns of 
      chromosomes 1, 4, 5, 9, 13, and 17 were almost identical in all six nodes, 
      indicating metastatic spread. In the second case, three of the nodes showed very 
      similar patterns of chromosome aberrations, including those of chromosomes 1, 4, 
      5, 7, 8, 10, 12, 14, 17, and 18. Aberrations of chromosomes 4, 5, 8, 10, 12, and 
      15 were shown in the fourth node, findings conclusive for both the metastatic 
      spread and the multicentric origin of the HCC. Based on the CGH results, five 
      probes, for chromosomes 1, 6, 7, 8, and X, were selected for FISH. Using this 
      panel, we found no aberrations in 14 HCAs. By contrast, 13/14 HCCs demonstrated 
      aberrations for two to five chromosomes in the FISH analysis. CONCLUSIONS: We 
      conclude that CGH and FISH are helpful diagnostic tools for the histopathological 
      differential diagnosis of HCC.
FAU - Wilkens, Ludwig
AU  - Wilkens L
AD  - Department of Pathology, Hannover Medical School, Carl-Neuberg-Strasse 1, 30625 
      Hannover, Germany.
FAU - Bredt, Martin
AU  - Bredt M
FAU - Flemming, Peer
AU  - Flemming P
FAU - Mengel, Michael
AU  - Mengel M
FAU - Becker, Thomas
AU  - Becker T
FAU - Klempnauer, Juergen
AU  - Klempnauer J
FAU - Kreipe, Hans
AU  - Kreipe H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Japan
TA  - J Hepatobiliary Pancreat Surg
JT  - Journal of hepato-biliary-pancreatic surgery
JID - 9431940
SB  - IM
MH  - Adenoma, Liver Cell/*diagnosis/genetics
MH  - Adult
MH  - Aged
MH  - Carcinoma, Hepatocellular/*diagnosis/genetics
MH  - Chromosome Aberrations
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Liver Neoplasms/*diagnosis/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Nucleic Acid Hybridization
MH  - Predictive Value of Tests
EDAT- 2002/09/28 04:00
MHDA- 2003/01/15 04:00
CRDT- 2002/09/28 04:00
PHST- 2002/09/28 04:00 [pubmed]
PHST- 2003/01/15 04:00 [medline]
PHST- 2002/09/28 04:00 [entrez]
AID - 10.1007/s005340200034 [doi]
PST - ppublish
SO  - J Hepatobiliary Pancreat Surg. 2002;9(3):304-11. doi: 10.1007/s005340200034.