PMID- 12353812
OWN - NLM
STAT- MEDLINE
DCOM- 20030129
LR  - 20190906
IS  - 0167-6806 (Print)
IS  - 0167-6806 (Linking)
VI  - 75
IP  - 3
DP  - 2002 Oct
TI  - Patterns of aneusomy for three chromosomes in individual cells from breast cancer 
      tumors.
PG  - 227-39
AB  - Multi-color fluorescence in situ hybridization (FISH) can determine the changes 
      in the copy numbers of several chromosomes simultaneously and can therefore be 
      used to identify aneusomic patterns in individual cells. Aneusomic patterns may 
      be useful for determining the malignant nature of rare epithelial cells in the 
      blood of cancer patients. Touch preparations from 74 primary breast tumors were 
      evaluated for aneusomy of chromosomes 1, 8 and 17 by tri-color-FISH. In the first 
      part of the analysis, percentages of aneusomy for individual chromosomes and 
      their combinations were determined. In the second part of the analysis, aneusomic 
      patterns for these three chromosomes were analyzed in individual tumor cells and 
      compared to aneusomic patterns observed in leukocytes and in individual cells 
      from benign and normal breast tissue to determine aneusomic patterns indicative 
      of malignancy. Ninety-two percentage of the primary breast carcinomas showed 
      aneusomy for one or more enumerator probes. Comparison with benign breast tissue 
      identified six aneusomic patterns in individual carcinoma cells indicative for 
      malignancy by statistical analysis and not observed in leukocytes. Hence, certain 
      patterns of aneusomy in individual cells involving chromosomes 1, 8 and 17 are 
      indicative of malignancy in individual breast tumor cells and may be useful for 
      determining malignancy of rare epithelial cells in the blood of breast cancer 
      patients.
FAU - Fehm, Tanja
AU  - Fehm T
AD  - Cancer Immunobiology Center, UT Southwestern Medical Center, Dallas, TX 
      75390-8576, USA.
FAU - Morrison, Larry
AU  - Morrison L
FAU - Saboorian, H
AU  - Saboorian H
FAU - Hynan, Linda
AU  - Hynan L
FAU - Tucker, Thomas
AU  - Tucker T
FAU - Uhr, Jonathan
AU  - Uhr J
LA  - eng
GR  - CA 78303/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Breast Cancer Res Treat
JT  - Breast cancer research and treatment
JID - 8111104
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Adenocarcinoma, Mucinous/genetics/pathology
MH  - *Aneuploidy
MH  - Biomarkers, Tumor/*genetics
MH  - Biopsy
MH  - Breast Neoplasms/*genetics/pathology
MH  - Carcinoma, Papillary/genetics/pathology
MH  - Chromosomes, Human, Pair 1/*genetics
MH  - Chromosomes, Human, Pair 17/*genetics
MH  - Chromosomes, Human, Pair 8/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Incidence
MH  - Interphase/genetics
MH  - Leukocytes/metabolism/pathology
MH  - Neoplasm Staging
MH  - Neoplasms, Ductal, Lobular, and Medullary/genetics/pathology
EDAT- 2002/10/02 04:00
MHDA- 2003/01/30 04:00
CRDT- 2002/10/02 04:00
PHST- 2002/10/02 04:00 [pubmed]
PHST- 2003/01/30 04:00 [medline]
PHST- 2002/10/02 04:00 [entrez]
AID - 10.1023/a:1019901010758 [doi]
PST - ppublish
SO  - Breast Cancer Res Treat. 2002 Oct;75(3):227-39. doi: 10.1023/a:1019901010758.