PMID- 12353828
OWN - NLM
STAT- MEDLINE
DCOM- 20021023
LR  - 20190721
IS  - 0163-2116 (Print)
IS  - 0163-2116 (Linking)
VI  - 47
IP  - 9
DP  - 2002 Sep
TI  - Lack of evidence for involvement of fetal microchimerism in pathogenesis of 
      primary biliary cirrhosis.
PG  - 1909-14
AB  - Microchimerism may be involved in the etiopathogenesis of autoimmune diseases 
      such as scleroderma. Primary biliary cirrhosis (PBC) shares some features with 
      scleroderma, including a female predominance and a histologic picture reminiscent 
      of chronic graft-versus-host disease. Our aim was to detect Y-chromosome-specific 
      sequences as a marker for microchimerism in liver tissue of female patients with 
      PBC. Liver biopsies of 105 female patients were investigated (28 patients with 
      primary biliary cirrhosis, 25 patients with chronic hepatitis C, 6 patients with 
      chronic hepatitis B, 9 with autoimmune hepatitis, and 37 patients with other 
      liver diseases) by a sensitive Y-chromosome-specific polymerase chain reaction 
      and/or fluorescence in situ hybridization (FISH) technique for the detection of 
      the Y chromosome on a single cell level. In the liver of 9 (8.6%) female patients 
      Y-chromosome-specific sequences were detected by PCR. Five of the patients had 
      PBC as underlying disease, 2 had chronic hepatitis C, and 2 other liver diseases. 
      No significant difference in the positivity rate for Y-specific sequences in 
      females with PBC and patients with other liver diseases was found (P > 0.05). By 
      FISH, single cells with one Y chromosome were detected in liver specimens from 3 
      of 21 patients suffering from PBC and from 1 of 13 patients with other liver 
      diseases. In summary, microchimerism can be detected in livers of patients with 
      hepatic diseases. However, in our study we found no evidence for an increased 
      prevalence of microchimerism in the livers of patients with primary biliary 
      cirrhosis. Our data suggest that microchimerism does not play a significant role 
      in the development of PBC.
FAU - Schoniger-Hekele, Maximilian
AU  - Schoniger-Hekele M
AD  - Universitatsklinik fur Innere Medizin IV, Klinische Abteilung Gastroenterologie 
      und Hepatologie, University of Vienna, Austria.
FAU - Muller, Christian
AU  - Muller C
FAU - Ackermann, Jutta
AU  - Ackermann J
FAU - Drach, Johannes
AU  - Drach J
FAU - Wrba, Friedrich
AU  - Wrba F
FAU - Penner, Edward
AU  - Penner E
FAU - Ferenci, Peter
AU  - Ferenci P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Dig Dis Sci
JT  - Digestive diseases and sciences
JID - 7902782
SB  - IM
MH  - Biopsy
MH  - *Chimera
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Liver/pathology/ultrastructure
MH  - Liver Cirrhosis, Biliary/etiology/*genetics
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction
MH  - Pregnancy
MH  - *Y Chromosome
EDAT- 2002/10/02 04:00
MHDA- 2002/10/31 04:00
CRDT- 2002/10/02 04:00
PHST- 2002/10/02 04:00 [pubmed]
PHST- 2002/10/31 04:00 [medline]
PHST- 2002/10/02 04:00 [entrez]
AID - 10.1023/a:1019623418063 [doi]
PST - ppublish
SO  - Dig Dis Sci. 2002 Sep;47(9):1909-14. doi: 10.1023/a:1019623418063.