PMID- 12354672
OWN - NLM
STAT- MEDLINE
DCOM- 20031003
LR  - 20190813
IS  - 0303-7207 (Print)
IS  - 0303-7207 (Linking)
VI  - 195
IP  - 1-2
DP  - 2002 Sep 30
TI  - Effect of estrogens on ontogenetic expression of progesterone receptor in the 
      fetal female rat reproductive tract.
PG  - 55-64
AB  - Ontogenetic expression of progesterone receptor (PR) and effect of estrogens on 
      PR expression in the fetal female rat reproductive tract were investigated. To 
      evaluate ontogenetic PR expression, female reproductive tract from untreated 
      fetuses was examined on gestational days (GD) 15.5, 17.5, 19.5 and 21.5. To 
      evaluate estrogen effects, pregnant rats were injected once per day with oil, 
      17beta-estradiol (E(2)) or diethylstilbestrol (DES) from GD 15 through 21. Female 
      fetuses were prepared for real-time reverse-transcription polymerase chain 
      reaction (RT-PCR) or immunohistochemistry for PR. Increase in PR mRNA expression 
      was detected in the Mullerian duct on GD 21.5 compared to that on GDs 15.5 and 
      17.5 in untreated fetuses (P<0.05). Prenatal administration of E(2) or DES 
      increased Mullerian PR mRNA levels by GD 21.5 compared with oil controls 
      (P<0.01). To identify cell and region in which PR was expressed and up-regulated 
      by E(2) and DES, localization was evaluated within three regions along the 
      Mullerian duct axis which differentiate into oviduct, uterus and upper vagina in 
      immunohistochemistry. In untreated fetuses, Mullerian epithelial PR 
      immunoreactivity was weak on GDs 15.5 and 17.5, but then became moderate on GDs 
      19.5 and 21.5 in all three regions. These fetuses exhibited faint signals in 
      Mullerian mesenchymal PR immunoreactivity during gestational monitoring. 
      Critically, Mullerian mesenchymal PR staining became intense after E(2) exposure 
      in all three regions by GD 21.5, but no change was observed in Mullerian 
      epithelial PR. Similarly, DES dramatically induced Mullerian mesenchymal PR in 
      all regions by GD 21.5, and also enhanced proximal epithelial PR. On the other 
      hand, middle and caudal epithelial PRs were reduced by DES. These affected 
      mesenchymal and epithelial cells by DES were ER alpha immunopositive in the 
      Mullerian duct, except for middle Mullerian epithelium. These findings clearly 
      demonstrate cell-specific PR localization and region-specific effect of DES on PR 
      in the developing rat Mullerian duct, and provide fundamental information 
      critical for investigating the tissue-specific mechanisms underlying the prenatal 
      response to estrogen receptor agonists.
FAU - Okada, Akinobu
AU  - Okada A
AD  - Safety Research Laboratories, Yamanouchi Pharmaceutical Co Ltd, Tokyo, Japan.
FAU - Ohta, Yasuhiko
AU  - Ohta Y
FAU - Buchanan, David
AU  - Buchanan D
FAU - Sato, Tomomi
AU  - Sato T
FAU - Iguchi, Taisen
AU  - Iguchi T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (Estrogens)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 731DCA35BT (Diethylstilbestrol)
SB  - IM
MH  - Animals
MH  - Diethylstilbestrol/pharmacology
MH  - Estradiol/pharmacology
MH  - Estrogens/*pharmacology
MH  - Female
MH  - Fetus
MH  - Gene Expression Regulation, Developmental/*drug effects
MH  - Genitalia, Female/cytology/embryology/*metabolism
MH  - Immunohistochemistry
MH  - Mullerian Ducts/cytology/growth & development/metabolism
MH  - Pregnancy
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Progesterone/*biosynthesis/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tissue Distribution
MH  - Up-Regulation
EDAT- 2002/10/02 04:00
MHDA- 2003/10/04 05:00
CRDT- 2002/10/02 04:00
PHST- 2002/10/02 04:00 [pubmed]
PHST- 2003/10/04 05:00 [medline]
PHST- 2002/10/02 04:00 [entrez]
AID - S0303720702002113 [pii]
AID - 10.1016/s0303-7207(02)00211-3 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2002 Sep 30;195(1-2):55-64. doi: 
      10.1016/s0303-7207(02)00211-3.