PMID- 12355207
OWN - NLM
STAT- MEDLINE
DCOM- 20030430
LR  - 20141120
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 111
IP  - 3
DP  - 2002 Sep
TI  - Isolation and identification of a novel satellite DNA family highly conserved in 
      several Cervidae species.
PG  - 176-83
AB  - In an attempt to amplify cervid satellite II DNA from the genomes of Indian 
      muntjac and Chinese muntjac, a pair of primers derived from the white tailed deer 
      satellite II DNA clone (OvDII) yielded a prominent approximately 1 kb polymerase 
      chain reaction (PCR) product (in addition to the expected 0.7 kb satellite II DNA 
      fragments) in both species. The approximately 1 kb products were cloned, 
      sequenced, and analyzed by Southern blotting and fluorescence in situ 
      hybridization (FISH). This revealed that the approximately 1 kb cloned sequences 
      indeed represent a previously unknown cervid satellite DNA family, which is now 
      designated as cervid satellite IV DNA. Approximately 1 kb PCR clones were also 
      obtained from the genomes of the black tailed deer and Canadian woodland caribou 
      with similar primer pairs. Extremely high sequence conservation (over 90% 
      homology) was observed among the clones generated from all four deer species and 
      PCR-Southern hybridization experiments further verified the co-amplification of 
      two kinds of satellite DNA sequences with the same pair of primers. This 
      satellite DNA was found to co-localize with centromeric proteins at the 
      kinetochore by a simultaneous FISH and immunofluorescence study. Due to its high 
      sequence conservation and close association with kinetochores, the newly 
      identified satellite DNA may have a functional centromeric role.
FAU - Li, Y C
AU  - Li YC
AD  - Department of Life Sciences, Chung Shan Medical University, No110, Sec1, Chien 
      Kuo N Rd, Taichung, Taiwan 40203.
FAU - Lee, C
AU  - Lee C
FAU - Chang, W S
AU  - Chang WS
FAU - Li, S-Y
AU  - Li SY
FAU - Lin, C C
AU  - Lin CC
LA  - eng
SI  - GENBANK/AY064466
SI  - GENBANK/AY064467
SI  - GENBANK/AY064468
SI  - GENBANK/AY064469
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020723
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chromosome Mapping
MH  - Conserved Sequence/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - In Vitro Techniques
MH  - Kinetochores/metabolism
MH  - Molecular Sequence Data
MH  - Muntjacs/*genetics
MH  - Polymerase Chain Reaction
MH  - Reindeer/genetics
MH  - Sequence Alignment
MH  - Sequence Analysis, DNA
EDAT- 2002/10/02 04:00
MHDA- 2003/05/06 05:00
CRDT- 2002/10/02 04:00
PHST- 2002/01/15 00:00 [received]
PHST- 2002/06/11 00:00 [accepted]
PHST- 2002/10/02 04:00 [pubmed]
PHST- 2003/05/06 05:00 [medline]
PHST- 2002/10/02 04:00 [entrez]
AID - 10.1007/s00412-002-0200-x [doi]
PST - ppublish
SO  - Chromosoma. 2002 Sep;111(3):176-83. doi: 10.1007/s00412-002-0200-x. Epub 2002 Jul 
      23.