PMID- 12355208
OWN - NLM
STAT- MEDLINE
DCOM- 20030430
LR  - 20061115
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 111
IP  - 3
DP  - 2002 Sep
TI  - The identification and analysis of the sequences that allow the detection of 
      Allium cepa chromosomes by GISH in the allodiploid A. wakegi.
PG  - 184-91
AB  - In Allium wakegi, which is an allodiploid species between Allium cepa and Allium 
      fistulosum, each genome can be clearly distinguished using genomic in situ 
      hybridization (GISH). Genomic DNA of A. cepa and A. fistulosum is differentiated 
      both qualitatively and quantitatively. We wanted to isolate nucleotide sequences 
      that give genome-specific signals on A. cepa chromosomes in GISH experiments in 
      A. wakegi. We isolated 23 clones that show GISH-like signal patterns in 
      fluorescence in situ hybridization (FISH) and analyzed their distribution in the 
      A. cepa- and A. fistulosum-derived genomes of A. wakegi. There was considerable 
      variation in the abundance and distribution of these cloned sequences on the 
      chromosomes of the two species. The degree of A. cepa specificity varied among 
      the clones. Twenty-two of the clones showed an even distribution over most 
      chromosome arms with some clustering in the pericentromeric regions, but one 
      clone showed very distinct terminal signals on some chromosomes. Whereas these 
      sequences are not specific for A. cepa, changes in bases in nucleotide sequences 
      and in their amount result in genome-specific characteristics in GISH 
      experiments.
FAU - Shibata, Fukashi
AU  - Shibata F
AD  - Biological Institute, Faculty of Science, Ehime University, Matsuyama 790-8577, 
      Japan. shibatafukashi@hotmail.com
FAU - Hizume, Masahiro
AU  - Hizume M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20020614
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
SB  - IM
MH  - Blotting, Southern
MH  - *Evolution, Molecular
MH  - Gene Dosage
MH  - In Situ Hybridization, Fluorescence
MH  - Onions/*genetics
MH  - *Repetitive Sequences, Nucleic Acid
EDAT- 2002/10/02 04:00
MHDA- 2003/05/06 05:00
CRDT- 2002/10/02 04:00
PHST- 2001/12/06 00:00 [received]
PHST- 2002/05/09 00:00 [revised]
PHST- 2002/05/15 00:00 [accepted]
PHST- 2002/10/02 04:00 [pubmed]
PHST- 2003/05/06 05:00 [medline]
PHST- 2002/10/02 04:00 [entrez]
AID - 10.1007/s00412-002-0197-1 [doi]
PST - ppublish
SO  - Chromosoma. 2002 Sep;111(3):184-91. doi: 10.1007/s00412-002-0197-1. Epub 2002 Jun 
      14.