PMID- 12355436
OWN - NLM
STAT- MEDLINE
DCOM- 20021127
LR  - 20131121
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 32
IP  - 10
DP  - 2002 Oct
TI  - Negative regulation of monocyte chemoattractant protein-1 gene expression by a 
      mouse estrogen-enhanced transcript.
PG  - 2837-46
AB  - A novel gene containing two typical estrogen responsive elements (ERE) was cloned 
      from MTEC1 cells, a mouse thymus epithelial cell line that produces 
      constitutively many chemokines. This gene is a homologue of the rat 
      estrogen-enhanced transcript (EET) gene, and is called the mEET gene. mEET 
      protein is expressed in cytoplasm. Addition of 17 beta-estradiol (E2) to the 
      MTEC1 cell cultures enhanced mEET mRNA expression and, meanwhile, significantly 
      inhibited monocyte chemoattractant protein-1 (MCP-1) production. To analyze the 
      functional links between the expression of mEET and of MCP-1, we transfected 
      MTEC1 cells with ERE-deleted antisense- or sense-mEET complementary (c)DNA 
      construct and activated the transfected mEET cDNA in stable MTEC1 transfectants 
      with doxycycline (Dox). Dox-induced activation of the mEET gene profoundly 
      inhibited MCP-1 expression at both mRNA and protein levels and alleviated its 
      chemotactic activity. Conversely, inactivation of the mEET gene substantially 
      augmented MCP-1 expression. Activation of the mEET gene markedly attenuated 
      activity of nuclear NF-kappaB. In summary, we have first demonstrated that 
      estrogen-imposed inhibition of MCP-1 expression occurs through the activation of 
      the mEET gene, its product suppresses nuclear NF-kappaB and negatively regulates 
      MCP-1 gene activation.
FAU - Xie, Lai-Ping
AU  - Xie LP
AD  - Department of Immunology, Peking University Health Science Centre, Beijing 100 
      083, China.
FAU - Fu, Wen-Xian
AU  - Fu WX
FAU - Jin, Cong
AU  - Jin C
FAU - Dong, Xue-Yuan
AU  - Dong XY
FAU - Chen, Wei-Feng
AU  - Chen WF
LA  - eng
SI  - GENBANK/AF171100
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Chemokine CCL2)
RN  - 0 (DNA, Complementary)
RN  - 0 (Litaf protein, rat)
RN  - 0 (NF-kappa B)
RN  - 0 (Phosphoproteins)
RN  - 0 (RNA, Messenger)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis/*genetics
MH  - DNA, Complementary/analysis
MH  - Estradiol/*pharmacology
MH  - Gene Expression Regulation/*drug effects
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Molecular Sequence Data
MH  - NF-kappa B/metabolism
MH  - Phosphoproteins
MH  - RNA, Messenger/analysis
MH  - Response Elements/*physiology
MH  - Transcription, Genetic
MH  - Transcriptional Activation
MH  - Transfection
EDAT- 2002/10/02 04:00
MHDA- 2002/11/28 04:00
CRDT- 2002/10/02 04:00
PHST- 2002/10/02 04:00 [pubmed]
PHST- 2002/11/28 04:00 [medline]
PHST- 2002/10/02 04:00 [entrez]
AID - 10.1002/1521-4141(2002010)32:10<2837::AID-IMMU2837>3.0.CO;2-V [doi]
PST - ppublish
SO  - Eur J Immunol. 2002 Oct;32(10):2837-46. doi: 
      10.1002/1521-4141(2002010)32:10<2837::AID-IMMU2837>3.0.CO;2-V.