PMID- 12356794
OWN - NLM
STAT- MEDLINE
DCOM- 20030415
LR  - 20220321
IS  - 0305-7453 (Print)
IS  - 0305-7453 (Linking)
VI  - 50
IP  - 4
DP  - 2002 Oct
TI  - Metallo-beta-lactamase-producing Enterobacteriaceae isolates in a university 
      hospital in Taiwan: prevalence of IMP-8 in Enterobacter cloacae and first 
      identification of VIM-2 in Citrobacter freundii.
PG  - 503-11
AB  - A total of 9082 clinical isolates of Enterobacteriaceae other than Klebsiella 
      spp. collected in 1999 and 2000 at a university hospital in Taiwan were 
      investigated for the production of metallo- beta-lactamases (MBLs). Thirty-six 
      (2.9%) of the 1261 Enterobacter cloacae isolates and one (0.3%) of the 340 
      Citrobacter freundii isolates were found to carry bla(IMP-8) and bla(VIM-2), 
      respectively, by colony hybridization, PCR and sequence analysis. The IMP-8 
      producers were recovered from 20 patients and four of them had recently 
      transferred from other hospitals, implying spread of IMP-8-producing E. cloacae 
      among different healthcare settings. Of the 20 non-repetitive IMP-8 producers, 17 
      (85%) isolates also harboured bla(SHV-12), which was on the same transferable 
      plasmids with bla(IMP-8). The bla(VIM-2)-positive isolate and all non-repetitive 
      bla(IMP-8)-positive isolates appeared susceptible to imipenem (MICs < 8 mg/L) and 
      meropenem (MICs < 4 mg/L), indicating the difficulty in detection of MBLs in 
      Enterobacteriaceae by routine susceptibility testing. Ribotyping of the 
      IMP-8-producing E. cloacae isolates indicated that the dissemination of 
      bla(IMP-8) was due largely to the spread of an epidemic clone, but horizontal 
      transfer among unrelated strains also occurred.
FAU - Yan, Jing-Jou
AU  - Yan JJ
AD  - Department of Pathology, College of Medicine, National Cheng Kung University, No. 
      1 University Road, Tainan 70101, Taiwan.
FAU - Ko, Wen-Chien
AU  - Ko WC
FAU - Chuang, Chin-Luan
AU  - Chuang CL
FAU - Wu, Jiunn-Jong
AU  - Wu JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Antimicrob Chemother
JT  - The Journal of antimicrobial chemotherapy
JID - 7513617
RN  - EC 3.5.2.- (IMP-8 enzyme)
RN  - EC 3.5.2.- (beta-lactamase bla(vim-2))
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Citrobacter freundii/*enzymology/genetics/isolation & purification
MH  - Enterobacter cloacae/*enzymology/genetics/isolation & purification
MH  - Enterobacteriaceae/enzymology/genetics/isolation & purification
MH  - Hospitals, University/statistics & numerical data
MH  - Humans
MH  - Microbial Sensitivity Tests/statistics & numerical data
MH  - Taiwan/epidemiology
MH  - beta-Lactamases/*biosynthesis/genetics
EDAT- 2002/10/03 04:00
MHDA- 2003/04/16 05:00
CRDT- 2002/10/03 04:00
PHST- 2002/10/03 04:00 [pubmed]
PHST- 2003/04/16 05:00 [medline]
PHST- 2002/10/03 04:00 [entrez]
AID - 10.1093/jac/dkf170 [doi]
PST - ppublish
SO  - J Antimicrob Chemother. 2002 Oct;50(4):503-11. doi: 10.1093/jac/dkf170.