PMID- 12357434
OWN - NLM
STAT- MEDLINE
DCOM- 20021107
LR  - 20061115
IS  - 0021-9967 (Print)
IS  - 0021-9967 (Linking)
VI  - 453
IP  - 1
DP  - 2002 Nov 4
TI  - Cerebellar Golgi, Purkinje, and basket cells have reduced gamma-aminobutyric acid 
      immunoreactivity in stargazer mutant mice.
PG  - 85-99
AB  - The stargazer mutant mouse has characteristic ataxia and head-tossing traits 
      coupled with a severe impairment in the acquisition of classical eye-blink 
      conditioning (Qiao et al. [1996] J. Neurosci. 16:640-648; Qiao et al. [ 1998] J. 
      Neurosci. 18:6990-6999). These phenotypes are thought to be cerebellar mediated 
      and have been attributed to the specific reduction in brain-derived neurotrophic 
      factor (BDNF). The granule cells in the cerebellum of the stargazer mouse exhibit 
      a near-total and exclusive ablation of BDNF mRNA expression and a consequent 
      defect in TrkB receptor signalling. To investigate whether the stargazer mutation 
      and lack of availability of BDNF in the granule cells compromise the phenotype of 
      the cerebellar inhibitory neurons, specifically their immunoreactivity for 
      gamma-aminobutyric acid (GABA); the levels of GABA neurotransmitter expressed in 
      Golgi, Purkinje, and basket cells; and the density of their synaptic contacts 
      were compared in stargazer and wild-type controls using electron microscopy and 
      quantitative immunogold labelling. The data presented in this study clearly show 
      that, in the spontaneous ataxic mutant mouse stargazer, the cerebellar inhibitory 
      neurons have significantly reduced levels of GABA immunoreactivity indicative of 
      a significant decrease in their GABA content compared with wild-type controls. 
      Furthermore, the density of inhibitory synapses between Golgi interneurons and 
      granule cells and also between basket and Purkinje cells in stargazer mutants is 
      reduced to approximately half that in wild-type controls. Whether this reduction 
      in GABA content and inhibitory synapse density is directly attributable to the 
      lack of BDNF in the cerebellum of the stargazer mutant is yet to be proved.
CI  - Copyright 2002 Wiley-Liss, Inc.
FAU - Richardson, Christine A
AU  - Richardson CA
AD  - Department of Biological and Biomedical Sciences, University of Durham, Durham 
      DH1 3LE, United Kingdom.
FAU - Leitch, Beulah
AU  - Leitch B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Comp Neurol
JT  - The Journal of comparative neurology
JID - 0406041
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/deficiency/genetics
MH  - Cell Communication/genetics/physiology
MH  - Cerebellar Ataxia/genetics/metabolism
MH  - Cerebellum/*metabolism/*ultrastructure
MH  - Immunohistochemistry
MH  - Interneurons/*metabolism/ultrastructure
MH  - Mice
MH  - Mice, Neurologic Mutants
MH  - Microscopy, Electron
MH  - Purkinje Cells/*metabolism/ultrastructure
MH  - Synapses/genetics/*ultrastructure
MH  - gamma-Aminobutyric Acid/*biosynthesis/genetics
EDAT- 2002/10/03 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/10/03 04:00
PHST- 2002/10/03 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/10/03 04:00 [entrez]
AID - 10.1002/cne.10406 [doi]
PST - ppublish
SO  - J Comp Neurol. 2002 Nov 4;453(1):85-99. doi: 10.1002/cne.10406.