PMID- 12359090
OWN - NLM
STAT- MEDLINE
DCOM- 20021017
LR  - 20191106
IS  - 1225-8687 (Print)
IS  - 1225-8687 (Linking)
VI  - 35
IP  - 5
DP  - 2002 Sep 30
TI  - Endotoxins of enteric pathogens are chemotactic factors for human neutrophils.
PG  - 482-7
AB  - Early activation of human peripheral blood polymorphonuclear neutrophils is 
      characterized by their morphological changes from spherical to polarized shapes. 
      The endotoxins from enteric pathogens (S. dysenteriae type 1, V. cholerae Inaba 
      569B, S. typhimurium, and K. pneumoniae) were assessed by their ability to induce 
      morphological polarization of the neutrophils as measures of early activation. 
      Phagocytic activity, adhesion, chemokinetic locomotion, and nitroblue tetrazolium 
      (NBT) dye-reduction ability measured the later activation of the cells. 
      Neutrophils showed distinct morphological polarization in suspension over a wide 
      range of concentrations of these endotoxins when were compared with those that 
      were induced by the standard chemotactic factor, 
      N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP). It was discovered that all 
      of the endotoxins induced locomotor responses in neutrophils in suspension that 
      were dose- and time-dependent. The optimum concentration for the endotoxins of S. 
      dysenteriae, V. cholerae, and K. pneumoniae was 1 mg/ml in which 71, 69, and 66% 
      of the neutrophils were polarized. However, the S. typhimurium dose was 2 mg/ml 
      in which 50% of the cells responded. Neutrophils that were stimulated with 
      endotoxins also showed increased random locomotion (p<0.005) through cellulose 
      nitrate filters, but an enhanced adhesion of the cells to glass surfaces 
      (p<0.03). These are important functions of these cells to reach and phagocytose 
      damaged cells, as well as invading microorganisms. Interestingly, the endotoxins 
      had a highly-significant inhibitory effect upon the proportions of neutrophils 
      phagocytosing opsonized yeast (p<0.01) with a small number of yeast that were 
      engulfed by the cells (p<0.02). Further, endotoxin-treated cells showed an 
      enhanced ability to reduce NBT dye (p<0.03). Therefore, we concluded that 
      endotoxins of enteric pathogens are neutrophil chemotactic factors.
FAU - Islam, Laila N
AU  - Islam LN
AD  - Department of Biochemistry and Molecular Biology, University of Dhaka, 
      Dhaka-1000, Bangladesh. laila@udhaka.net
FAU - Nabi, A H M Nurun
AU  - Nabi AH
FAU - Ahmed, K Mokim
AU  - Ahmed KM
FAU - Sultana, Novera
AU  - Sultana N
LA  - eng
PT  - Journal Article
PL  - Korea (South)
TA  - J Biochem Mol Biol
JT  - Journal of biochemistry and molecular biology
JID - 9702084
RN  - 0 (Chemotactic Factors)
RN  - 0 (Endotoxins)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Movement
MH  - Chemotactic Factors/*physiology
MH  - Chemotaxis
MH  - Endotoxins/*physiology
MH  - Humans
MH  - In Vitro Techniques
MH  - Klebsiella pneumoniae/*physiology
MH  - Lipopolysaccharides
MH  - Neutrophils/*metabolism/physiology
MH  - Phagocytosis
MH  - Shigella dysenteriae/*physiology
MH  - Time Factors
MH  - Vibrio cholerae/*physiology
EDAT- 2002/10/03 04:00
MHDA- 2002/10/18 04:00
CRDT- 2002/10/03 04:00
PHST- 2002/10/03 04:00 [pubmed]
PHST- 2002/10/18 04:00 [medline]
PHST- 2002/10/03 04:00 [entrez]
AID - 10.5483/bmbrep.2002.35.5.482 [doi]
PST - ppublish
SO  - J Biochem Mol Biol. 2002 Sep 30;35(5):482-7. doi: 10.5483/bmbrep.2002.35.5.482.