PMID- 12359144
OWN - NLM
STAT- MEDLINE
DCOM- 20030527
LR  - 20191106
IS  - 1096-7192 (Print)
IS  - 1096-7192 (Linking)
VI  - 77
IP  - 1-2
DP  - 2002 Sep-Oct
TI  - Genetic network identification by high density, multiplexed reversed 
      transcriptional (HD-MRT) analysis in steroidogenic axis model cell lines.
PG  - 159-78
AB  - Transcriptional network analysis in steroidogenic axis cell lines requires an 
      understanding of cellular network composition and complexity. Previous studies 
      have shown that absence of transcriptional network components in a cell line 
      compromises that cell line's functional capacity for transcriptional regulation. 
      Our goal was to analyze qualitatively steroidogenic axis-derived cell lines' 
      expression of a putative transcriptional network involved in human and mouse 
      development. To pursue this analysis we used Northern blots and a high 
      density-multiplexed reverse transcription-polymerase chain reaction (HD-MRT-PCR) 
      approach. Our results revealed that, while some members of this putative network 
      were universally expressed, only a minority of the non-constitutive targeted 
      transcripts were present in any single line. Based on our data and previously 
      published results for contextual expression of these transcription factors, a 
      model was constructed possessing the topology suggestive of a scale-free network: 
      certain network members were highly connected nodes and would represent critical 
      sites of vulnerability. The importance of these highly connected nodes for 
      network function is supported by the severe phenotypes exhibited by human 
      patients and animal models when these genes are mutated. We conclude that 
      knowledge of network composition in specific cell lines is essential for their 
      use as models to investigate functional interactions within selected subnetworks.
FAU - Clipsham, R
AU  - Clipsham R
AD  - UCLA Molecular Biology Institute, Los Angeles, CA, USA.
FAU - Zhang, Y H
AU  - Zhang YH
FAU - Huang, B L
AU  - Huang BL
FAU - McCabe, E R B
AU  - McCabe ER
LA  - eng
GR  - P30 HD34610/HD/NICHD NIH HHS/United States
GR  - R01 HD39322/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Genet Metab
JT  - Molecular genetics and metabolism
JID - 9805456
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (Steroids)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Alternative Splicing
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Line
MH  - DNA Primers/genetics
MH  - DNA, Complementary/genetics
MH  - Gene Expression Profiling
MH  - Humans
MH  - Mice
MH  - *Models, Genetic
MH  - Mutation
MH  - Polymerase Chain Reaction/*methods
MH  - RNA/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Steroids/*biosynthesis
MH  - *Transcription, Genetic
EDAT- 2002/10/03 04:00
MHDA- 2003/05/28 05:00
CRDT- 2002/10/03 04:00
PHST- 2002/10/03 04:00 [pubmed]
PHST- 2003/05/28 05:00 [medline]
PHST- 2002/10/03 04:00 [entrez]
AID - S1096719202001191 [pii]
AID - 10.1016/s1096-7192(02)00119-1 [doi]
PST - ppublish
SO  - Mol Genet Metab. 2002 Sep-Oct;77(1-2):159-78. doi: 10.1016/s1096-7192(02)00119-1.