PMID- 12359151
OWN - NLM
STAT- MEDLINE
DCOM- 20021119
LR  - 20191106
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 21
IP  - 1
DP  - 2002 Sep
TI  - BDNF modulates sensory neuron synaptic activity by a facilitation of GABA 
      transmission in the dorsal horn.
PG  - 51-62
AB  - Topical application of brain-derived neurotrophic factor (BDNF) to the adult rat 
      isolated dorsal horn with dorsal root attached preparation inhibited the 
      electrically evoked release of substance P (SP) from sensory neurons. This effect 
      of BDNF was dose dependent (EC(50) 250 pM) and reversed by the tyrosine kinase 
      inhibitor, K-252a. BDNF-induced inhibition of SP release was blocked by the 
      GABA(B) receptor antagonist CGP 55485 but not by naloxone. Acute application of 
      BDNF significantly increased potassium-stimulated release of GABA in the dorsal 
      horn isolated in vitro and this effect was blocked by K-252a. Intrathecal 
      injection of BDNF into the rat lumbar spinal cord induced a short-lasting 
      increase in hindpaw threshold to noxious thermal stimulation that was blocked by 
      CGP 55485 and was associated with activation of ERK in dorsal horn. These data 
      suggest that exogenous BDNF can indirectly modulate primary sensory neuron 
      synaptic efficacy via facilitation of the release of GABA from dorsal horn 
      interneurons.
FAU - Pezet, Sophie
AU  - Pezet S
AD  - Neuroscience Research Centre, Guy's, King's and St Thomas' School of Biomedical 
      Sciences, King's College London, London SE1 1UL, United Kingdom.
FAU - Cunningham, Joanna
AU  - Cunningham J
FAU - Patel, Jaykumar
AU  - Patel J
FAU - Grist, John
AU  - Grist J
FAU - Gavazzi, Isabella
AU  - Gavazzi I
FAU - Lever, Isobel J
AU  - Lever IJ
FAU - Malcangio, Marzia
AU  - Malcangio M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Narcotic Antagonists)
RN  - 0 (Receptors, Opioid)
RN  - 33507-63-0 (Substance P)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Afferent Pathways/*drug effects/metabolism
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism/*pharmacology
MH  - Cells, Cultured
MH  - Ganglia, Spinal/*drug effects/metabolism
MH  - Injections, Spinal
MH  - Male
MH  - Narcotic Antagonists/pharmacology
MH  - Neural Inhibition/drug effects/physiology
MH  - Pain/*drug therapy/metabolism/physiopathology
MH  - Pain Threshold/drug effects/physiology
MH  - Posterior Horn Cells/*drug effects/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reaction Time/drug effects/physiology
MH  - Receptors, Opioid/metabolism
MH  - Substance P/metabolism
MH  - Synaptic Transmission/*drug effects/physiology
MH  - gamma-Aminobutyric Acid/*metabolism/pharmacology
EDAT- 2002/10/03 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/10/03 04:00
PHST- 2002/10/03 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/10/03 04:00 [entrez]
AID - S1044743102911668 [pii]
AID - 10.1006/mcne.2002.1166 [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 2002 Sep;21(1):51-62. doi: 10.1006/mcne.2002.1166.