PMID- 12359346
OWN - NLM
STAT- MEDLINE
DCOM- 20030106
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 187
IP  - 1-2
DP  - 2002 Dec 10
TI  - Profiling of human cytokines in healthy individuals with vitamin E 
      supplementation by antibody array.
PG  - 17-24
AB  - Previously, we demonstrated that vitamin E supplementation decreases 
      autoantibodies to oxidized lipid-protein complexes (J. Med. Food 1 (2000) 247). 
      Utilizing an in vitro modeling system, we also demonstrated that vitamin E blocks 
      the tumor promotion process in liver epithelial cells (Carcinogenesis 20 (1999) 
      485 and Mol. Carcinog. 30 (2001) 209). To investigate the molecular mechanisms of 
      vitamin E function, we developed a human cytokine array system that is capable of 
      detecting the expression of 35 cytokines simultaneously. Using this new 
      technology, we analyzed the potential vitamin E-regulated cytokines in vitamin E 
      supplementation individuals. The cytokine arrays showed that expression of 
      several cytokines, particularly monocyte chemoattractant protein-1 (MCP-1), was 
      profoundly reduced in vitamin E supplementation individuals. Moreover, addition 
      of vitamin E to several cultured cells significantly down-regulated the 
      expression of MCP-1. Our results suggested that MCP-1 may be one of the most 
      important targets of antioxidant vitamin E. To the best of our knowledge, this is 
      the first report describing the down-regulation of MCP-1 in vitamin E 
      supplementation in vivo.
FAU - Lin, Ying
AU  - Lin Y
AD  - Department of Gynecology and Obstetrics, Emory University School of Medicine, 
      1639 Pierce Drive, Room 429, Atlanta, GA 30322, USA.
FAU - Huang, Ruochun
AU  - Huang R
FAU - Santanam, Nalini
AU  - Santanam N
FAU - Liu, Ya-Guang
AU  - Liu YG
FAU - Parthasarathy, Sampath
AU  - Parthasarathy S
FAU - Huang, Ruo-Pan
AU  - Huang RP
LA  - eng
GR  - CA89273/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (Antioxidants)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin G)
RN  - 1406-18-4 (Vitamin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antioxidants/*administration & dosage
MH  - Chemokine CCL2/metabolism
MH  - Cytokines/*metabolism
MH  - Diet
MH  - Dietary Supplements
MH  - Down-Regulation
MH  - Gene Expression Profiling
MH  - Humans
MH  - Immunoassay/methods
MH  - Immunoglobulin G/immunology
MH  - In Vitro Techniques
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - Vitamin E/*administration & dosage
EDAT- 2002/10/03 04:00
MHDA- 2003/01/08 04:00
CRDT- 2002/10/03 04:00
PHST- 2002/10/03 04:00 [pubmed]
PHST- 2003/01/08 04:00 [medline]
PHST- 2002/10/03 04:00 [entrez]
AID - S0304383502003464 [pii]
AID - 10.1016/s0304-3835(02)00346-4 [doi]
PST - ppublish
SO  - Cancer Lett. 2002 Dec 10;187(1-2):17-24. doi: 10.1016/s0304-3835(02)00346-4.