PMID- 12359763
OWN - NLM
STAT- MEDLINE
DCOM- 20021108
LR  - 20041117
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 62
IP  - 19
DP  - 2002 Oct 1
TI  - Identification of novel cryptic translocations involving IGH in B-cell 
      non-Hodgkin's lymphomas.
PG  - 5523-7
AB  - Chromosomal rearrangements involving the immunoglobulin heavy chain gene (IGH) at 
      14q32 are observed in approximately 50% of patients with B-cell non-Hodgkin's 
      lymphoma (NHL). The 5' end of the IGH gene is located within 8 kb of the 
      telomeric repeats of 14q. Translocations involving the IGH locus and the 
      telomeric band of a partner chromosome are difficult to identify, because most 
      terminal bands of human chromosomes appear pale by conventional G-banding 
      techniques. To determine whether there are cryptic translocations involving the 
      IGH locus, we used dual-color fluorescence in situ hybridization (FISH) of 5' and 
      3' IGH genomic clones containing the variable sequences, or the J(H) and the 5' 
      constant regions, respectively. We examined cells from 51 patients with B-cell 
      NHL who had a normal karyotype (3 patients), clonal abnormalities not involving 
      14q32 (35 patients), or alterations of 14q32 other than recurring translocations, 
      i.e., add(14)(q32) (13 patients). FISH detected 17 IGH translocations in 16 of 51 
      (31%) cases. Of the 13 cases with add(14)(q32), FISH identified the partner 
      chromosome in 9 cases (69%; 3q27, 6 cases; 2p13, 19p13.3, and 18q21.3, 1 case 
      each). Six of thirty-eight (16%) patients without visible alterations of 14q32 
      and 2 of 13 (15%) patients with an abnormality of one chromosome 14 had masked (5 
      patients) or cryptic IGH translocations (3 patients), involving 3q27 (3 
      patients), 5p15.3 (2 patients), 19p13.3 (3 patients), or 14q32 (1 patient; 1 
      patient had two rearrangements). We identified two novel, recurring, cryptic 
      translocations: t(5;14)(p15.3;q32) (2 patients) and t(14;19)(q32;p13.3) (3 
      patients). In summary, FISH permitted the detection of cryptic or masked IGH 
      rearrangements in approximately 20% of lymphoma cases without visible 
      rearrangements of 14q32 analyzed retrospectively.
FAU - Gozzetti, Alessandro
AU  - Gozzetti A
AD  - Section of Hematology/Oncology, Departments of Medicine, University of Chicago, 
      Chicago, Illinois 60637, USA.
FAU - Davis, Elizabeth M
AU  - Davis EM
FAU - Espinosa, Rafael 3rd
AU  - Espinosa R 3rd
FAU - Fernald, Anthony A
AU  - Fernald AA
FAU - Anastasi, John
AU  - Anastasi J
FAU - Le Beau, Michelle M
AU  - Le Beau MM
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child, Preschool
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Chromosomes, Human, Pair 5/genetics
MH  - Female
MH  - Genes, Immunoglobulin/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell/*genetics/immunology/pathology
MH  - Male
MH  - Middle Aged
MH  - Translocation, Genetic
EDAT- 2002/10/03 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/10/03 04:00
PHST- 2002/10/03 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/10/03 04:00 [entrez]
PST - ppublish
SO  - Cancer Res. 2002 Oct 1;62(19):5523-7.