PMID- 12362050
OWN - NLM
STAT- MEDLINE
DCOM- 20030304
LR  - 20131121
IS  - 0289-0003 (Print)
IS  - 0289-0003 (Linking)
VI  - 19
IP  - 9
DP  - 2002 Sep
TI  - Distribution of NO-induced cGMP-like immunoreactive neurones in the abdominal 
      nervous system of the crayfish, Procambarus clarkii.
PG  - 969-79
AB  - Nitric oxide (NO) acts as a signalling molecule by activating soluble guanylate 
      cyclase and causing accumulation of the second messenger cyclic guanosine 
      3',5'-monophosphate (cGMP) in target cells. In order to detect the presence of 
      NO-cGMP signalling pathway in the crayfish abdominal nervous system, accumulation 
      of NO-induced cGMP was investigated by anti-cGMP immunochemistry. Some 
      preparations were incubated in a high-K(+) saline containing an inhibitor of 
      cGMP-degrading phosphodiesterase, 3-isobutyl-1-methyxanthine (IBMX), to activate 
      NO generating neurones, which could release NO in the ganglion, and then 
      immunohistochemistry using an anti-cGMP antibody was performed. The other 
      preparations were incubated in NO donor, sodium nitroprusside (SNP) saline 
      containing IBMX before anti-cGMP immunohistochemistry was performed. The 
      distribution of cGMP-like immunoreactive neurones in high-K(+) treated 
      preparations was similar to that of cGMP-like immunoreactive neurones in NO donor 
      treated preparations. About 70-80 cell bodies and many neuronal branches in the 
      neuropilar area of the ganglion were stained, although no neurones showed 
      immunoreactivity unless preparations were activated by either high-K(+) or the NO 
      donor. Some of them were identical neurones, and they were intersegmental 
      ascending interneurones and motor neurones. Sensory afferents that innervates 
      hind gut showed strong cGMP-like immunoreactivity, although no mechanosensory 
      afferents showed any immunoreactivity. These results strongly suggest the 
      presence of an NO-cGMP signalling pathway that regulates neuronal events in the 
      abdominal nervous system of the crayfish.
FAU - Aonuma, Hitoshi
AU  - Aonuma H
AD  - Laboratory of Neuro-Cybernetics, Research Institute for Electronic Science, 
      Hokkaido University, Sapporo, Japan. aon@ncp8.es.hokudai.ac.jp
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Zoolog Sci
JT  - Zoological science
JID - 8702287
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - H2D2X058MU (Cyclic GMP)
RN  - RWP5GA015D (Potassium)
RN  - TBT296U68M (1-Methyl-3-isobutylxanthine)
SB  - IM
MH  - 1-Methyl-3-isobutylxanthine/pharmacology
MH  - Abdomen/*innervation
MH  - Animals
MH  - *Astacoidea/cytology/drug effects/immunology
MH  - Cyclic GMP/immunology/*metabolism
MH  - Female
MH  - Ganglia/cytology/drug effects/metabolism
MH  - Immunohistochemistry
MH  - Male
MH  - Nervous System/*cytology/drug effects/*metabolism
MH  - Neurons/drug effects/immunology/*metabolism
MH  - Nitric Oxide/*metabolism
MH  - Potassium/pharmacology
MH  - Signal Transduction/drug effects
EDAT- 2002/10/04 04:00
MHDA- 2003/03/05 04:00
CRDT- 2002/10/04 04:00
PHST- 2002/10/04 04:00 [pubmed]
PHST- 2003/03/05 04:00 [medline]
PHST- 2002/10/04 04:00 [entrez]
AID - 10.2108/zsj.19.969 [doi]
PST - ppublish
SO  - Zoolog Sci. 2002 Sep;19(9):969-79. doi: 10.2108/zsj.19.969.