PMID- 12363468
OWN - NLM
STAT- MEDLINE
DCOM- 20021209
LR  - 20190826
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 26
IP  - 11
DP  - 2002 Nov
TI  - Increased circulating normal and BCR-ABL+Ve progenitor numbers in Philadelphia 
      chromosome-positive acute myeloid leukaemia.
PG  - 997-1005
AB  - We recorded elevated numbers of circulating myeloid and erythroid colony-forming 
      cells in 15 adult patients with acute myeloid leukaemia (AML) who presented with 
      high blood white cell counts. Since leukaemic blasts from three of these patients 
      were Philadelphia chromosome-positive (Ph+), we were able to determine if blood 
      progenitors from these particular patients arose from the leukaemic clone or from 
      residual normal progenitors. Blasts and colonies were intensively investigated 
      using a combination of cell surface marker analysis by flow cytometry, RT-PCR and 
      interphase fluorescence in situ hybridization (FISH). FISH detected 
      rearrangements within the major breakpoint BCR (M-BCR) region in blasts and in 
      some myeloid and erythroid colonies from patients 1 and 2. The minor breakpoint 
      (m-BCR) region was detected in blasts and in some myeloid and erythroid colonies 
      from patient 3. RT-PCR detected long b2a2 BCR-ABL transcripts in blasts from 
      patients 1 and 2, although misspliced short e1a2 transcripts were also seen in 
      patient 1. Only e1a2 transcripts were found in blasts from patient 3. Flow 
      sorting demonstrated the B-cell marker CD19 on blasts and on a proportion of 
      myeloid and erythroid progenitors from patients 1 and 3. RT-PCR also detected IgH 
      rearrangements, further evidence of B-cell differentiation, in blasts from these 
      two patients. We conclude that both normal and clonal circulating progenitor 
      numbers can be raised in both M-BCR and m-BCR Ph+ AML. The underlying cause, 
      perhaps efflux from a congested marrow, may be common to AML patients with a high 
      blood white cell count.
FAU - Baines, Paul
AU  - Baines P
AD  - Haematology Department, University Hospital of Wales, CF14 4XW, Cardiff, UK. 
      bainesp@cf.ac.uk
FAU - Austin, Steve
AU  - Austin S
FAU - Fisher, Janet
AU  - Fisher J
FAU - Owen-Jones, Eleri
AU  - Owen-Jones E
FAU - Lee-Jones, Lisa
AU  - Lee-Jones L
FAU - Throp, Duncan
AU  - Throp D
FAU - Mckinley, Mark
AU  - Mckinley M
FAU - Hoy, Terry
AU  - Hoy T
FAU - Mills, Ken
AU  - Mills K
FAU - Thompson, Peter
AU  - Thompson P
FAU - Burnett, Alan
AU  - Burnett A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
RN  - EC 2.7.10.1 (Protein-Tyrosine Kinases)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
RN  - EC 2.7.11.1 (BCR protein, human)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-bcr)
SB  - IM
CIN - Leuk Res. 2002 Nov;26(11):971-3. PMID: 12363461
MH  - Acute Disease
MH  - Adult
MH  - Biomarkers, Tumor
MH  - Case-Control Studies
MH  - Colony-Forming Units Assay
MH  - DNA, Neoplasm/analysis
MH  - Erythroid Precursor Cells/*metabolism
MH  - Flow Cytometry
MH  - Fusion Proteins, bcr-abl/blood/*genetics
MH  - Genes, Immunoglobulin/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*genetics/metabolism
MH  - Leukocyte Count
MH  - Myeloid Progenitor Cells/*metabolism
MH  - Oncogene Proteins/genetics/metabolism
MH  - *Protein-Tyrosine Kinases
MH  - *Proto-Oncogene Proteins
MH  - Proto-Oncogene Proteins c-bcr
MH  - RNA, Messenger/genetics
MH  - RNA, Neoplasm/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2002/10/05 04:00
MHDA- 2002/12/10 04:00
CRDT- 2002/10/05 04:00
PHST- 2002/10/05 04:00 [pubmed]
PHST- 2002/12/10 04:00 [medline]
PHST- 2002/10/05 04:00 [entrez]
AID - S0145212602000498 [pii]
AID - 10.1016/s0145-2126(02)00049-8 [doi]
PST - ppublish
SO  - Leuk Res. 2002 Nov;26(11):997-1005. doi: 10.1016/s0145-2126(02)00049-8.