PMID- 12364978
OWN - NLM
STAT- MEDLINE
DCOM- 20030303
LR  - 20081121
IS  - 1008-682X (Print)
IS  - 1008-682X (Linking)
VI  - 4
IP  - 3
DP  - 2002 Sep
TI  - Studies on the integration of hepatitis B virus DNA sequence in human sperm 
      chromosomes.
PG  - 209-12
AB  - AIM: To study the integration of hepatitis B virus (HBV) DNA into sperm 
      chromosomes in hepatitis B patients and the features of its integration. METHODS: 
      Sperm chromosomes of 14 subjects (5 healthy controls and 9 HB patients, including 
      1 acute hepatitis B, 2 chronic active hepatitis B, 4 chronic persistent hepatitis 
      B, 2 HBsAg chronic carriers with no clinical symptoms) were prepared using 
      interspecific in vitro fertilization between zona-free hamster oocytes and human 
      spermatozoa. Fluorescence in situ hybridization (FISH) to sperm chromosome 
      spreads was carried out with biotin-labeled full length HBV DNA probe to detect 
      the specific HBV DNA sequences in the sperm chromosomes. RESULTS: Specific 
      fluorescent signal spots for HBV DNA were seen in sperm chromosomes of one 
      patient with chronic persistent hepatitis B. In 9 (9/42) sperm chromosome 
      complements containing fluorescent signal spots, one presented 5 obvious FISH 
      spots and the others 2 to 4 signals. The fluorescence intensity showed 
      significant difference among the signal spots. The distribution of signal sites 
      among chromosomes seems to be random. CONCLUSION: HBV could integrate into human 
      sperm chromosomes. Results suggest that the possibility of vertical transmission 
      of HBV via the germ line to the next generation is present.
FAU - Huang, Jian-Min
AU  - Huang JM
AD  - Department of Cell Biology and Medical Genetics, Shantou University Medical 
      College, Shantou 515031, China. thhuang@stu.edu.cn
FAU - Huang, Tian-Hua
AU  - Huang TH
FAU - Qiu, Huan-Ying
AU  - Qiu HY
FAU - Fang, Xiao-Wu
AU  - Fang XW
FAU - Zhuang, Tian-Gang
AU  - Zhuang TG
FAU - Qiu, Jie-Wen
AU  - Qiu JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Asian J Androl
JT  - Asian journal of andrology
JID - 100942132
SB  - IM
MH  - Adult
MH  - Chromosomes, Human/genetics/virology
MH  - Hepatitis B/genetics/transmission/*virology
MH  - Hepatitis B virus/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infectious Disease Transmission, Vertical
MH  - Male
MH  - Spermatozoa/*virology
MH  - *Virus Integration
EDAT- 2002/10/05 04:00
MHDA- 2003/03/04 04:00
CRDT- 2002/10/05 04:00
PHST- 2002/10/05 04:00 [pubmed]
PHST- 2003/03/04 04:00 [medline]
PHST- 2002/10/05 04:00 [entrez]
PST - ppublish
SO  - Asian J Androl. 2002 Sep;4(3):209-12.