PMID- 12366750
OWN - NLM
STAT- MEDLINE
DCOM- 20021127
LR  - 20191106
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 4
IP  - 10
DP  - 2002 Oct
TI  - Analysis of the microbial community structure and function of a laboratory scale 
      enhanced biological phosphorus removal reactor.
PG  - 559-69
AB  - A laboratory scale sequencing batch reactor (SBR) operating for enhanced 
      biological phosphorus removal (EBPR) and fed with a mixture of volatile fatty 
      acids (VFAs) showed stable and efficient EBPR capacity over a four-year-period. 
      Phosphorus (P), poly-beta-hydroxyalkanoate (PHA) and glycogen cycling consistent 
      with classical anaerobic/aerobic EBPR were demonstrated with the order of 
      anaerobic VFA uptake being propionate, acetate then butyrate. The SBR was 
      operated without pH control and 63.67 +/- 13.86 mg P l-1 was released 
      anaerobically. The P% of the sludge fluctuated between 6% and 10% over the 
      operating period (average of 8.04 +/- 1.31%). Four main morphological types of 
      floc-forming bacteria were observed in the sludge during one year of in-tensive 
      microscopic observation. Two of them were mainly responsible for 
      anaerobic/aerobic P and PHA transformations. Fluorescence in situ hybridization 
      (FISH) and post-FISH chemical staining for intracellular polyphosphate and PHA 
      were used to determine that 'Candidatus Accumulibacter phosphatis' was the most 
      abundant polyphosphate accumulating organism (PAO), forming large clusters of 
      coccobacilli (1.0-1.5 micro m) and comprising 53% of the sludge bacteria. Also by 
      these methods, large coccobacillus-shaped gammaproteobacteria (2.5-3.5 micro m) 
      from a recently described novel cluster were glycogen-accumulating organisms 
      (GAOs) comprising 13% of the bacteria. Tetrad-forming organisms (TFOs) consistent 
      with the 'G bacterium' morphotype were alphaproteobacteria, but not Amaricoccus 
      spp., and comprised 25% of all bacteria. According to chemical staining, TFOs 
      were occasionally able to store PHA anaerobically and utilize it aerobically.
FAU - Levantesi, Caterina
AU  - Levantesi C
AD  - Water Research Institute, Via Reno 1, 00198 Rome, Italy.
FAU - Serafim, Luisa S
AU  - Serafim LS
FAU - Crocetti, Gregory R
AU  - Crocetti GR
FAU - Lemos, Paulo C
AU  - Lemos PC
FAU - Rossetti, Simona
AU  - Rossetti S
FAU - Blackall, Linda L
AU  - Blackall LL
FAU - Reis, Maria A M
AU  - Reis MA
FAU - Tandoi, Valter
AU  - Tandoi V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Sewage)
RN  - 27YLU75U4W (Phosphorus)
RN  - 9005-79-2 (Glycogen)
SB  - IM
MH  - Bacteria, Anaerobic/*physiology
MH  - Biodegradation, Environmental
MH  - Glycogen/metabolism
MH  - Phosphorus/*metabolism
MH  - *Refuse Disposal
MH  - Sewage/*microbiology
EDAT- 2002/10/09 04:00
MHDA- 2002/11/28 04:00
CRDT- 2002/10/09 04:00
PHST- 2002/10/09 04:00 [pubmed]
PHST- 2002/11/28 04:00 [medline]
PHST- 2002/10/09 04:00 [entrez]
AID - 339 [pii]
AID - 10.1046/j.1462-2920.2002.00339.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2002 Oct;4(10):559-69. doi: 10.1046/j.1462-2920.2002.00339.x.