PMID- 12366778
OWN - NLM
STAT- MEDLINE
DCOM- 20030725
LR  - 20190826
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 60
IP  - 1
DP  - 2002 Jul
TI  - Analysis of the T-cell receptor beta-chain variable-region (Vbeta) repertoire in 
      chronic human Chagas' disease.
PG  - 10-5
AB  - Previous studies have shown that infection with the protozoan Trypanosoma cruzi 
      (Chagas' disease) is associated with genetic components [human leukocyte antigen 
      (HLA) genes and T-cell receptor (TCR) genes]. We studied the TCR Vbeta repertoire 
      of peripheral blood lymphocytes of 23 unrelated serologically positive subjects 
      using reverse transcriptase-polymerase chain reaction (RT-PCR). The patients, 
      previously tested for HLA genotypes, were clinically classified as asymptomatic, 
      arrhythmic and cardiopathic patients. Statistical analysis showed the significant 
      increment of the Vbeta7 family in chagasics with arrhythmia compared with 
      asymptomatic and cardiopathic patients, indicating that the frequency of this 
      family is variable in different clinical forms of the disease and possibly that 
      these T cells might be a marker of the progression of Chagas' disease. Based on 
      the calculation of a Delta score the order of variability in the TCR repertoire 
      was: patients with heart failure > asymptomatic > arrhythmic patients. The major 
      histocompatibility complex (MHC) of the individual may influence the use of 
      particular V genes in T-cell response to foreign antigens. We found a significant 
      increase of the Vbeta7 family in arrhythmic patients who were DRB1*01 DQB1*0501 
      DPB1*0401, a marker associated with susceptibility to cardiac damage in Chagas' 
      disease. If confirmed by further studies in a larger cohort, a possible 
      association between the TCR Vbeta repertoire and the MHC haplotype of chagasic 
      patients could be postulated.
FAU - Fernandez-Mestre, M T
AU  - Fernandez-Mestre MT
AD  - Laboratorio de Fisiopatologia, Centro de Medicina Experimental, Instituto 
      Venezolano de Investigaciones Cientificas, Caracas, Venezuela.
FAU - Jaraquemada, D
AU  - Jaraquemada D
FAU - Bruno, R E
AU  - Bruno RE
FAU - Caro, J
AU  - Caro J
FAU - Layrisse, Z
AU  - Layrisse Z
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA Antigens)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Alleles
MH  - Arrhythmias, Cardiac/genetics/metabolism
MH  - Chagas Disease/genetics/*metabolism
MH  - Chronic Disease
MH  - Gene Frequency/genetics
MH  - Genotype
MH  - HLA Antigens/genetics
MH  - Heart Failure/genetics/metabolism
MH  - Humans
MH  - Leukocytes, Mononuclear/metabolism
MH  - Middle Aged
MH  - Receptors, Antigen, T-Cell, alpha-beta/genetics/*metabolism
MH  - Statistics as Topic
MH  - Venezuela
RF  - 25
EDAT- 2002/10/09 04:00
MHDA- 2003/07/26 05:00
CRDT- 2002/10/09 04:00
PHST- 2002/10/09 04:00 [pubmed]
PHST- 2003/07/26 05:00 [medline]
PHST- 2002/10/09 04:00 [entrez]
AID - tan600102 [pii]
AID - 10.1034/j.1399-0039.2002.600102.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2002 Jul;60(1):10-5. doi: 10.1034/j.1399-0039.2002.600102.x.