PMID- 12367753
OWN - NLM
STAT- MEDLINE
DCOM- 20021213
LR  - 20190910
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 89
IP  - 1
DP  - 2002 Oct
TI  - Identification and characterization of the gene products of open reading frame 
      U86/87 of human herpesvirus 6.
PG  - 89-101
AB  - The human herpesvirus 6 (HHV-6) immediate early-A locus (IE-A) locates in the 
      position analogous to the human cytomegalovirus (HCMV) major IE (MIE) locus that 
      is well-known to play critical roles in viral infection. Similarly to HCMV MIE, 
      HHV-6 IE-A consists of two genetic units, IE1 and IE2, corresponding to open 
      reading frames U90-U89 and U90-U86/87, respectively. However, the HHV-6 IE-A 
      locus exhibits limited sequence homology with the HCMV MIE locus. In this study, 
      to characterize HHV-6 IE2 gene products, polyclonal antibodies against four 
      domains of the U86/87 open reading frame were generated by immunization of 
      rabbits with bacterially-expressed proteins. Three polypeptides derived from the 
      U86/87 region with apparent molecular masses of 100, 85 and 55 kD were detected 
      in HHV-6-infected cells 3 days after infection, while IE1 polypeptides with 
      apparent molecular mass greater than 170 kD were detectable as early as 8 h. 
      Mapping of the IE2 gene products with the antibodies suggests differential 
      splicing and alternative translation initiation in the IE2 genetic unit. The IE2 
      products show a mixed cytoplasmic and nuclear localization pattern. In addition, 
      the 437 amino acid carboxyl-terminus domain bound to a DNA fragment containing 
      the putative IE-A promoter. These results suggest that HHV-6 IE2 plays a critical 
      role in transcriptional regulation and viral growth as does HCMV IE2, although it 
      is likely that HHV-6 IE2 has expression kinetics different from HCMV IE2.
FAU - Papanikolaou, Eleni
AU  - Papanikolaou E
AD  - Laboratory of General Microbiology, Section of Genetics, Developmental and 
      Molecular Biology, School of Biology, Aristotle Universit, Thessaloniki 54006, 
      Greece.
FAU - Kouvatsis, Vlassis
AU  - Kouvatsis V
FAU - Dimitriadis, Georgios
AU  - Dimitriadis G
FAU - Inoue, Naoki
AU  - Inoue N
FAU - Arsenakis, Minas
AU  - Arsenakis M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Antibodies, Viral)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Immediate-Early Proteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood/immunology
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/metabolism
MH  - Fetal Blood/cytology
MH  - *Gene Expression Regulation, Viral
MH  - Herpesvirus 6, Human/genetics/growth & development/*metabolism
MH  - Humans
MH  - *Immediate-Early Proteins/chemistry/genetics/immunology/metabolism
MH  - Immunization
MH  - Immunoblotting
MH  - Lymphocytes/virology
MH  - Open Reading Frames/*genetics
MH  - Rabbits
MH  - Recombinant Fusion Proteins/immunology
MH  - Transcription, Genetic
MH  - Viral Proteins/chemistry/genetics/immunology/metabolism
EDAT- 2002/10/09 04:00
MHDA- 2002/12/17 04:00
CRDT- 2002/10/09 04:00
PHST- 2002/10/09 04:00 [pubmed]
PHST- 2002/12/17 04:00 [medline]
PHST- 2002/10/09 04:00 [entrez]
AID - S0168170202001260 [pii]
AID - 10.1016/s0168-1702(02)00126-0 [doi]
PST - ppublish
SO  - Virus Res. 2002 Oct;89(1):89-101. doi: 10.1016/s0168-1702(02)00126-0.