PMID- 12368315
OWN - NLM
STAT- MEDLINE
DCOM- 20021126
LR  - 20191210
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 76
IP  - 21
DP  - 2002 Nov
TI  - Hantavirus infection of dendritic cells.
PG  - 10724-33
AB  - Dendritic cells (DCs) play a pivotal role as antigen-presenting cells in the 
      antiviral immune response. Here we show that Hantaan virus (HTNV), which belongs 
      to the Bunyaviridae family (genus Hantavirus) and causes hemorrhagic fever with 
      renal syndrome, productively infects human DCs in vitro. In the course of HTNV 
      infection, DCs did not show any cytopathic effect and viral replication did not 
      induce cell lysis or apoptosis. Furthermore, HTNV did not affect 
      apoptosis-inducing signals that are important for the homeostatic control of 
      mature DCs. In contrast to immunosuppressive viruses, e.g., human 
      cytomegalovirus, HTNV activated immature DCs, resulting in upregulation of major 
      histocompatibility complex (MHC), costimulatory, and adhesion molecules. 
      Intriguingly, strong upregulation of MHC class I molecules and an increased 
      intercellular cell adhesion molecule type 1 expression was also detected on 
      HTNV-infected endothelial cells. In addition, antigen uptake by HTNV-infected DCs 
      was reduced, another characteristic feature of DC maturation. Consistent with 
      these findings, we observed that HTNV-infected DCs stimulated T cells as 
      efficiently as did mature DCs. Finally, infection of DCs with HTNV induced the 
      release of the proinflammatory cytokines tumor necrosis factor alpha and alpha 
      interferon. Taken together, our findings indicate that hantavirus-infected DCs 
      may significantly contribute to hantavirus-associated pathogenesis.
FAU - Raftery, Martin J
AU  - Raftery MJ
AD  - Institute of Virology, Charite Medical School, Humboldt University Berlin, 
      D-10098 Berlin, Germany.
FAU - Kraus, Annette A
AU  - Kraus AA
FAU - Ulrich, Rainer
AU  - Ulrich R
FAU - Kruger, Detlev H
AU  - Kruger DH
FAU - Schonrich, Gunther
AU  - Schonrich G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Antigens, CD)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (CD86 protein, human)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 187348-17-0 (Interleukin-12)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antigens, CD/biosynthesis
MH  - B7-1 Antigen/biosynthesis
MH  - B7-2 Antigen
MH  - CD40 Antigens/biosynthesis
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Dendritic Cells/*immunology/virology
MH  - Hantaan virus/*immunology
MH  - Histocompatibility Antigens Class I/biosynthesis
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/biosynthesis
MH  - Interferon-gamma/metabolism
MH  - Interleukin-12/metabolism
MH  - Membrane Glycoproteins/biosynthesis
MH  - Tumor Necrosis Factor-alpha/metabolism
MH  - Up-Regulation
MH  - Vero Cells
PMC - PMC136604
EDAT- 2002/10/09 04:00
MHDA- 2002/11/28 04:00
PMCR- 2002/11/01
CRDT- 2002/10/09 04:00
PHST- 2002/10/09 04:00 [pubmed]
PHST- 2002/11/28 04:00 [medline]
PHST- 2002/10/09 04:00 [entrez]
PHST- 2002/11/01 00:00 [pmc-release]
AID - 0507 [pii]
AID - 10.1128/jvi.76.21.10724-10733.2002 [doi]
PST - ppublish
SO  - J Virol. 2002 Nov;76(21):10724-33. doi: 10.1128/jvi.76.21.10724-10733.2002.