PMID- 12368494
OWN - NLM
STAT- MEDLINE
DCOM- 20030123
LR  - 20240410
IS  - 1040-4651 (Print)
IS  - 1532-298X (Electronic)
IS  - 1040-4651 (Linking)
VI  - 14
IP  - 10
DP  - 2002 Oct
TI  - Phosphatidylinositol 3- and 4-phosphate are required for normal stomatal 
      movements.
PG  - 2399-412
AB  - Phosphatidylinositol (PI) metabolism plays a central role in signaling pathways 
      in both animals and higher plants. Stomatal guard cells have been reported to 
      contain PI 3-phosphate (PI3P) and PI 4-phosphate (PI4P), the products of PI 
      3-kinase (PI3K) and PI 4-kinase (PI4K) activities. In this study, we tested the 
      roles of PI3P and PI4P in stomatal movements. Both wortmannin (WM) and LY294002 
      inhibited PI3K and PI4K activities in guard cells and promoted stomatal opening 
      induced by white light or the circadian clock. WM and LY294002 also inhibited 
      stomatal closing induced by abscisic acid (ABA). Furthermore, overexpression in 
      guard cells of GFP:EBD (green fluorescent protein:endosome binding domain of 
      human EEA1) or GFP:FAPP1PH (PI-four-P adaptor protein-1 pleckstrin homology 
      domain), which bind to PI3P and PI4P, respectively, increased stomatal apertures 
      under darkness and white light and partially inhibited stomatal closing induced 
      by ABA. The reduction in ABA-induced stomatal closing with reduced levels of PI 
      monophosphate seemed to be attributable, at least in part, to impaired Ca(2+) 
      signaling, because WM and LY294002 inhibited ABA-induced cytosolic Ca(2+) 
      increases in guard cells. These results suggest that PI3P and PI4P play an 
      important role in the modulation of stomatal closing and that reductions in the 
      levels of functional PI3P and PI4P enhance stomatal opening.
FAU - Jung, Ji-Yul
AU  - Jung JY
AD  - Division of Molecular Life Sciences, Pohang University of Science and Technology, 
      Pohang 790-784, Korea.
FAU - Kim, Yong-Woo
AU  - Kim YW
FAU - Kwak, June M
AU  - Kwak JM
FAU - Hwang, Jae-Ung
AU  - Hwang JU
FAU - Young, Jared
AU  - Young J
FAU - Schroeder, Julian I
AU  - Schroeder JI
FAU - Hwang, Inhwan
AU  - Hwang I
FAU - Lee, Youngsook
AU  - Lee Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Plant Cell
JT  - The Plant cell
JID - 9208688
RN  - 0 (Androstadienes)
RN  - 0 (Blood Proteins)
RN  - 0 (Chromones)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Morpholines)
RN  - 0 (Phosphatidylinositol Phosphates)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Phosphoproteins)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (phosphatidylinositol 3-phosphate)
RN  - 0 (phosphatidylinositol 4-phosphate)
RN  - 0 (platelet protein P47)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - 72S9A8J5GW (Abscisic Acid)
RN  - EC 2.7.1.67 (1-Phosphatidylinositol 4-Kinase)
RN  - SY7Q814VUP (Calcium)
RN  - XVA4O219QW (Wortmannin)
SB  - IM
EIN - Plant Cell. 2002 Nov;14(11):2975.
MH  - 1-Phosphatidylinositol 4-Kinase/metabolism
MH  - Abscisic Acid/pharmacology
MH  - Androstadienes/pharmacology
MH  - Arabidopsis/physiology
MH  - Blood Proteins/chemistry/metabolism
MH  - Calcium/metabolism
MH  - Calcium Signaling/drug effects
MH  - Chromones/pharmacology
MH  - Circadian Rhythm/drug effects
MH  - Darkness
MH  - Endosomes/chemistry/metabolism
MH  - Gene Expression Regulation, Plant/radiation effects
MH  - Green Fluorescent Proteins
MH  - Light
MH  - Luminescent Proteins/genetics/metabolism
MH  - Morpholines/pharmacology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphatidylinositol Phosphates/antagonists & inhibitors/*metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phosphoproteins/chemistry/metabolism
MH  - Plant Epidermis/cytology/*physiology
MH  - Plant Leaves/cytology/*physiology
MH  - Protein Binding/drug effects
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Signal Transduction
MH  - Vicia/physiology
MH  - Wortmannin
PMC - PMC151225
EDAT- 2002/10/09 04:00
MHDA- 2003/01/24 04:00
PMCR- 2003/10/01
CRDT- 2002/10/09 04:00
PHST- 2002/10/09 04:00 [pubmed]
PHST- 2003/01/24 04:00 [medline]
PHST- 2002/10/09 04:00 [entrez]
PHST- 2003/10/01 00:00 [pmc-release]
AID - 004143 [pii]
AID - 10.1105/tpc.004143 [doi]
PST - ppublish
SO  - Plant Cell. 2002 Oct;14(10):2399-412. doi: 10.1105/tpc.004143.