PMID- 12368805
OWN - NLM
STAT- MEDLINE
DCOM- 20021205
LR  - 20231213
IS  - 1097-6256 (Print)
IS  - 1097-6256 (Linking)
VI  - 5
IP  - 11
DP  - 2002 Nov
TI  - BDNF release from single cells elicits local dendritic growth in nearby neurons.
PG  - 1177-84
AB  - In cultured neurons, the exogenous application of neurotrophins (in homogenous 
      concentrations) alters many features of axonal and dendritic arbors. In vivo, 
      however, release of endogenous neurotrophins from neuronal processes creates 
      spatially heterogeneous neurotrophin distributions. To probe the consequences of 
      such endogenous neurotrophin distribution, we produced 'donor neurons' in ferret 
      cortex brain slices that co-expressed brain-derived neurotrophic factor (BDNF) 
      and red fluorescent protein (RFP). Using two-photon microscopy, we analyzed their 
      effects on 'recipient neurons' that expressed green fluorescent protein (GFP) 
      alone. BDNF released from dendrites and cell bodies acted directly on nearby 
      recipient neurons to increase dendritic branching in a distance-dependent manner. 
      Three-dimensional analysis of donor and recipient dendrites indicated that the 
      BDNF source had to be within 4.5 microm to induce dendritic growth in the 
      recipient neuron. Thus, BDNF released from an individual cell alters the 
      structure of nearby dendrites on an exquisitely local scale.
FAU - Horch, Hadley Wilson
AU  - Horch HW
AD  - Howard Hughes Medical Institute, Department of Neurobiology, Duke University 
      Medical Center, Box 3209, Durham, North Carolina 27710, USA. hhorch@bowdoin.edu
FAU - Katz, Lawrence C
AU  - Katz LC
LA  - eng
GR  - EY-11553/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Nat Neurosci
JT  - Nature neuroscience
JID - 9809671
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Luminescent Proteins)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cells, Cultured
MH  - Dendrites/*physiology
MH  - Ferrets
MH  - Gene Expression
MH  - Luminescent Proteins/genetics
MH  - Microscopy, Fluorescence
MH  - Neural Pathways/*physiology
MH  - Neurons/*metabolism/ultrastructure
MH  - Visual Cortex/cytology
MH  - Red Fluorescent Protein
EDAT- 2002/10/09 04:00
MHDA- 2002/12/06 04:00
CRDT- 2002/10/09 04:00
PHST- 2002/07/15 00:00 [received]
PHST- 2002/09/09 00:00 [accepted]
PHST- 2002/10/09 04:00 [pubmed]
PHST- 2002/12/06 04:00 [medline]
PHST- 2002/10/09 04:00 [entrez]
AID - nn927 [pii]
AID - 10.1038/nn927 [doi]
PST - ppublish
SO  - Nat Neurosci. 2002 Nov;5(11):1177-84. doi: 10.1038/nn927.