PMID- 12370129
OWN - NLM
STAT- MEDLINE
DCOM- 20030114
LR  - 20131121
IS  - 1167-1122 (Print)
IS  - 1167-1122 (Linking)
VI  - 12
IP  - 5
DP  - 2002 Sep-Oct
TI  - Interleukin-4 induces apoptosis in cultured human follicular keratinocytes, but 
      not in dermal papilla cells.
PG  - 432-8
AB  - The importance of apoptosis in hair follicle cycling is still not clearly 
      understood, however, its regulation in follicular keratinocytes (FK) during bulb 
      regression (catagen) may be essential for hair regrowth. So far, the control of 
      FK apoptosis remains unknown. In this study, the anti-inflammatory cytokine IL-4 
      was found to induce apoptosis dose and time dependently in cultured human FK, in 
      contrast to other agents known to inhibit hair growth such as IL-1alpha, 
      IL-1beta, TNFalpha and TGFbeta, as shown by DNA fragmentation. On the other hand, 
      cytokines reported to be involved in hair follicle cycling including IL-4 were 
      not able to induce apoptosis in dermal papilla cells (DPC), in contrast to 
      staurosporine. This PKC inhibitor revealed dose-dependent apoptotic signals not 
      only for DPC but also for FK in vitro. In further experiments the expression of 
      apoptosis regulating proteins, possibly involved in catagen formation, was 
      analyzed in FK and DPC. However, no striking difference in RNA expression was 
      seen in either cell population under culture conditions and after incubation with 
      IL-4. We conclude, therefore, that IL-4 mediated apoptosis may participate in 
      regulating catagen formation in the hair follicle, acting selectively on cultured 
      FK and being independent of bcl-2 and bax expression.
FAU - Mandt, Nathalie
AU  - Mandt N
AD  - Department of Dermatology, University Medical Center Benjamin Franklin, The Free 
      University of Berlin, Fabeckstr. 60-62, 14195 Berlin, Germany. 
      nathalie.mandt@medizin.fu-berlin.de
FAU - Geilen, Christoph C
AU  - Geilen CC
FAU - Wrobel, Anna
AU  - Wrobel A
FAU - Gelber, Alexandra
AU  - Gelber A
FAU - Kamp, Hartwig
AU  - Kamp H
FAU - Orfanos, Constantin E
AU  - Orfanos CE
FAU - Blume-Peytavi, Ulrike
AU  - Blume-Peytavi U
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Dermatol
JT  - European journal of dermatology : EJD
JID - 9206420
RN  - 0 (Cytokines)
RN  - 207137-56-2 (Interleukin-4)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - H88EPA0A3N (Staurosporine)
SB  - IM
MH  - Apoptosis/*drug effects/physiology
MH  - Base Sequence
MH  - Cells, Cultured
MH  - Cytokines/pharmacology
MH  - Dermis/cytology/drug effects
MH  - Epidermal Growth Factor/pharmacology
MH  - Female
MH  - Fibroblasts/*drug effects/*physiology
MH  - Hair Follicle/cytology/drug effects
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Interleukin-4/*pharmacology
MH  - Keratinocytes/*drug effects/*physiology
MH  - Male
MH  - Molecular Sequence Data
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Staurosporine/pharmacology
EDAT- 2002/10/09 04:00
MHDA- 2003/01/15 04:00
CRDT- 2002/10/09 04:00
PHST- 2002/10/09 04:00 [pubmed]
PHST- 2003/01/15 04:00 [medline]
PHST- 2002/10/09 04:00 [entrez]
PST - ppublish
SO  - Eur J Dermatol. 2002 Sep-Oct;12(5):432-8.