PMID- 12371168
OWN - NLM
STAT- MEDLINE
DCOM- 20030206
LR  - 20190922
IS  - 0047-2425 (Print)
IS  - 0047-2425 (Linking)
VI  - 31
IP  - 5
DP  - 2002 Sep-Oct
TI  - Treatment of 2,4-dichlorophenol polluted soil with free and immobilized laccase.
PG  - 1509-15
AB  - Enzyme treatment is currently considered for remediation of terrestrial systems 
      polluted with organic compounds. In this study, two soils from Pennsylvania with 
      2.8 or 7.4% organic matter contents (Soils 1 and 2, respectively) were amended 
      with 14C-labeled 2,4-dichlorophenol (2,4-DCP) and incubated with a laccase from 
      Trametes villosa (free or immobilized on montmorillonite). 2,4-DCP was either 
      transformed to methanol-soluble polymeric products (11-32%) or covalently bound 
      to soil organic matter (53-85%); unaltered 2,4-DCP could be recovered from soil 
      by methanol extraction (0-38%) at the completion of a 14-d incubation period. In 
      Soil 1, both free and immobilized laccase removed 100% of 2,4-DCP without regard 
      for moisture conditions. In Soil 2, immobilized laccase removed more 2,4-DCP 
      (about 95%, regardless of moisture conditions) than free enzyme (55, 75, and 90% 
      at 30, 55, and 100% of maximum water-holding capacity, respectively). Binding of 
      2,4-DCP in the humin fraction was nearly the same for free and immobilized 
      laccase. More 2,4-DCP, however, was bound to humic and fulvic acids in the 
      presence of immobilized laccase than in the presence of free laccase. In general, 
      immobilized laccase performed better than free laccase. However, for practical 
      applications, the higher activity of immobilized laccase is offset by a 23% loss 
      in enzyme activity during immobilization, which approximates the 30% increase in 
      free laccase needed to achieve the same level of remediation. Furthermore, 
      immobilized laccase is more costly than free T. villosa laccase.
FAU - Ahn, Mi-Youn
AU  - Ahn MY
AD  - Laboratory of Soil Biochemistry, Pennsylvania State University, 129 Land and 
      Water, University Park, PA 16802, USA.
FAU - Dec, Jerzy
AU  - Dec J
FAU - Kim, Jang-Eok
AU  - Kim JE
FAU - Bollag, Jean-Marc
AU  - Bollag JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Environ Qual
JT  - Journal of environmental quality
JID - 0330666
RN  - 0 (Anthelmintics)
RN  - 0 (Chlorophenols)
RN  - 0 (Soil Pollutants)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.10.3.2 (Laccase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Anthelmintics/*metabolism
MH  - Chlorophenols/*metabolism
MH  - Laccase
MH  - Oxidoreductases/*pharmacology
MH  - Polyporales/enzymology
MH  - Soil Pollutants/*metabolism
EDAT- 2002/10/10 04:00
MHDA- 2003/02/07 04:00
CRDT- 2002/10/10 04:00
PHST- 2002/10/10 04:00 [pubmed]
PHST- 2003/02/07 04:00 [medline]
PHST- 2002/10/10 04:00 [entrez]
AID - 10.2134/jeq2002.1509 [doi]
PST - ppublish
SO  - J Environ Qual. 2002 Sep-Oct;31(5):1509-15. doi: 10.2134/jeq2002.1509.