PMID- 12372015
OWN - NLM
STAT- MEDLINE
DCOM- 20021209
LR  - 20240312
IS  - 0953-816X (Print)
IS  - 1460-9568 (Electronic)
IS  - 0953-816X (Linking)
VI  - 16
IP  - 5
DP  - 2002 Sep
TI  - Connexin29 expression, immunocytochemistry and freeze-fracture replica immunogold 
      labelling (FRIL) in sciatic nerve.
PG  - 795-806
AB  - The recently discovered connexin29 (Cx29) was reported to be present in the 
      central and peripheral nervous systems (CNS and PNS), and its mRNA was found in 
      particular abundance in peripheral nerve. The expression and localization of Cx29 
      protein in sciatic nerve were investigated using an antibody against Cx29. The 
      antibody recognized Cx29 in HeLa cells transfected with Cx29 cDNA, while 
      nontransfected HeLa cells were devoid of Cx29. Immunoblotting of sciatic nerve 
      homogenate revealed monomeric and possibly higher molecular weight forms of Cx29. 
      These were distinguished from connexin32 (Cx32), which also is expressed in 
      peripheral nerve. Double immunofluorescence labelling for Cx29 and Cx32 revealed 
      only partial colocalization of the two connexins, with codistribution at 
      intermittent, conical-shaped striations along nerve fibers. By freeze-fracture 
      replica immunogold labelling (FRIL), Cx32 was found in gap junctions in the 
      outermost layers of myelin, whereas Cx29-immunogold labelling was found only in 
      the innermost layer of myelin in close association with hexagonally arranged 
      intramembrane particle (IMP) 'rosettes' and gap junction-like clusters of IMPs. 
      Although both Cx32 and Cx29 were detected in myelin of normal mice, only Cx29 was 
      present in Schwann cell membranes in Cx32 knockout mice. The results confirm that 
      Cx29 is a second connexin expressed in Schwann cells of sciatic nerve. In 
      addition, Cx29 is present in distinctive IMP arrays in the inner most layer of 
      myelin, adjacent to internodal axonal plasma membranes, where this connexin may 
      have previously unrecognized functions.
FAU - Li, Xinbo
AU  - Li X
AD  - Department of Physiology, Faculty of Medicine, University of Manitoba, 730 
      William Avenue, Winnipeg, Manitoba, R3E 3J7 Canada.
FAU - Lynn, B D
AU  - Lynn BD
FAU - Olson, C
AU  - Olson C
FAU - Meier, C
AU  - Meier C
FAU - Davidson, K G V
AU  - Davidson KG
FAU - Yasumura, T
AU  - Yasumura T
FAU - Rash, J E
AU  - Rash JE
FAU - Nagy, J I
AU  - Nagy JI
LA  - eng
GR  - NS31027/NS/NINDS NIH HHS/United States
GR  - R01 NS038121/NS/NINDS NIH HHS/United States
GR  - R01 NS044395/NS/NINDS NIH HHS/United States
GR  - R01 NS044010/NS/NINDS NIH HHS/United States
GR  - NS38121/NS/NINDS NIH HHS/United States
GR  - NS39040/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Connexins)
RN  - 0 (Gje1 protein, mouse)
RN  - 0 (Nerve Tissue Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Connexins/*analysis/immunology
MH  - *Freeze Fracturing
MH  - Gap Junctions/chemistry
MH  - *Immunohistochemistry
MH  - Mice
MH  - Mice, Knockout
MH  - Myelin Sheath/chemistry
MH  - Nerve Tissue Proteins
MH  - Schwann Cells/chemistry
MH  - Sciatic Nerve/*chemistry
MH  - Gap Junction beta-1 Protein
PMC - PMC1803218
MID - NIHMS17744
EDAT- 2002/10/10 04:00
MHDA- 2002/12/10 04:00
PMCR- 2008/01/31
CRDT- 2002/10/10 04:00
PHST- 2002/10/10 04:00 [pubmed]
PHST- 2002/12/10 04:00 [medline]
PHST- 2002/10/10 04:00 [entrez]
PHST- 2008/01/31 00:00 [pmc-release]
AID - 2149 [pii]
AID - 10.1046/j.1460-9568.2002.02149.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2002 Sep;16(5):795-806. doi: 10.1046/j.1460-9568.2002.02149.x.