PMID- 12372466
OWN - NLM
STAT- MEDLINE
DCOM- 20021104
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 78
IP  - 4
DP  - 2002 Oct
TI  - Induction of monocyte chemotactic protein-1 in peritoneal mesothelial and 
      endometrial cells by oxidized low-density lipoprotein and peritoneal fluid from 
      women with endometriosis.
PG  - 843-8
AB  - OBJECTIVE: To elucidate the effect of oxidized low-density lipoprotein (LDL) and 
      peritoneal fluid of women with endometriosis on monocyte chemotactic protein-1 
      (MCP-1) production by peritoneal mesothelial cells and endometrial cells. DESIGN: 
      In vitro study. SETTING: University medical center. PATIENT(S): Five women 
      undergoing surgery for pelvic pain, infertility, or endometriosis; five women 
      without endometriosis who were undergoing tubal ligation were the controls. 
      INTERVENTION(S): Mesothelial cells and endometrial cells in culture were treated 
      with oxidized LDL and peritoneal fluid from control and endometriosis patients, 
      then MCP-1 levels were measured. MAIN OUTCOME MEASURE(S): ELISA was used to 
      measure MCP-1 in the culture supernatants exposed to oxidized LDL and peritoneal 
      fluid from control and endometriosis patients. Cellular MCP-1 messenger RNA 
      expression was evaluated by reverse transcription-polymerase chain reaction 
      (RT-PCR) assay. RESULT(S): Treatment with oxidized LDL caused an increase in 
      accumulation of immunoreactive MCP-1 in the medium of cultured mesothelial and 
      endometrial cells (primary endometrial stromal cells and endometrial cell line 
      EM42). The mesothelial cells secreted more MCP-1 than did endometrial cells under 
      the culture condition. The EM42 cells cultured in the presence of peritoneal 
      fluid from endometriosis patients secreted more MCP-1 than those cultured with 
      peritoneal fluid from normal women. However, no differences were found in MCP-1 
      levels in the supernatant of endometrial stromal cells cultured with peritoneal 
      fluid. CONCLUSION(S): This is the first report of MCP-1 expression in mesothelial 
      cells induced by oxidized LDL, and provides direct evidence of inflammatory 
      action of peritoneal fluid of women with endometriosis.
FAU - Rong, Rong
AU  - Rong R
AD  - Emory Center for Advanced Research on Women's Health, Department of Gynecology 
      and Obstetrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA.
FAU - Ramachandran, Sumathi
AU  - Ramachandran S
FAU - Santanam, Nalini
AU  - Santanam N
FAU - Murphy, Ana A
AU  - Murphy AA
FAU - Parthasarathy, Sampath
AU  - Parthasarathy S
LA  - eng
GR  - P01 HD35276/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (RNA, Messenger)
RN  - 0 (oxidized low density lipoprotein)
SB  - IM
MH  - Ascitic Fluid/*physiopathology
MH  - Cells, Cultured
MH  - Chemokine CCL2/analysis/*biosynthesis
MH  - Culture Media, Conditioned/chemistry
MH  - Endometriosis/*metabolism/pathology
MH  - Endometrium/drug effects/*metabolism/pathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelium/drug effects/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - Lipoproteins, LDL/*pharmacology
MH  - Oxidative Stress
MH  - Peritoneum/drug effects/metabolism/pathology
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stromal Cells/drug effects/metabolism/pathology
EDAT- 2002/10/10 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/10/10 04:00
PHST- 2002/10/10 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/10/10 04:00 [entrez]
AID - S0015028202033332 [pii]
AID - 10.1016/s0015-0282(02)03333-2 [doi]
PST - ppublish
SO  - Fertil Steril. 2002 Oct;78(4):843-8. doi: 10.1016/s0015-0282(02)03333-2.