PMID- 12372952
OWN - NLM
STAT- MEDLINE
DCOM- 20030312
LR  - 20171101
IS  - 1660-8151 (Print)
IS  - 1660-8151 (Linking)
VI  - 92
IP  - 3
DP  - 2002
TI  - In vitro effects of Habu snake venom on cultured mesangial cells.
PG  - 665-72
AB  - BACKGROUND: Habu snake venom (HSV)-induced glomerulonephritis is a unique model 
      showing a progressive course of mesangial proliferation. To elucidate the in 
      vitro effects of HSV, we examined whether HSV itself could have direct effects on 
      the cultured mesangial cells, such as cell proliferation and activation of 
      chemokine gene expression. METHODS: The incorporation of 
      5-[(125)I]iodo-2'-deoxyuridine was measured with a gamma-counter, and gene 
      expressions of growth factors, chemokines and cytokines were evaluated by a real 
      time quantitative PCR. RESULTS: We demonstrated that excessive or continuous HSV 
      stimulation decreased a mesangial cell viability. However, adequate and temporary 
      HSV stimulation induced proliferation of mesangial cells in vitro along with a 
      significant elevation of monocyte chemoattractant protein-1 (MCP-1) mRNA levels. 
      In addition to these in vitro results, we showed that MCP-1 mRNA levels increased 
      in renal cortices of glomerulonephritis induced by HSV. Immunohistochemistry also 
      showed a positive staining for MCP-1 in the marginal area of glomerulus with 
      mesangiolysis. CONCLUSIONS: These data suggest that HSV itself may elicit direct 
      biological effects on mesangial cells which may participate in pathophysiology of 
      glomerulonephritis induced by HSV.
CI  - Copyright 2002 S. Karger AG, Basel
FAU - Kubo, Atsushi
AU  - Kubo A
AD  - Department of Public Health, Nara Medical University, Kashihara, Nara, Japan.
FAU - Iwano, Masayuki
AU  - Iwano M
FAU - Kobayashi, Yoshiyuki
AU  - Kobayashi Y
FAU - Kyoda, Yusuke
AU  - Kyoda Y
FAU - Isumi, Yoshitaka
AU  - Isumi Y
FAU - Maruyama, Naoki
AU  - Maruyama N
FAU - Samejima, Kenichi
AU  - Samejima K
FAU - Dohi, Yoshiko
AU  - Dohi Y
FAU - Minamino, Naoto
AU  - Minamino N
FAU - Yonemasu, Kunio
AU  - Yonemasu K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Nephron
JT  - Nephron
JID - 0331777
RN  - 0 (Chemokine CCL2)
RN  - 0 (Crotalid Venoms)
RN  - 0 (Growth Substances)
RN  - 0 (Interleukin-1)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cell Division/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/analysis/genetics
MH  - Crotalid Venoms/*pharmacology
MH  - DNA/biosynthesis
MH  - Gene Expression/drug effects
MH  - Glomerular Mesangium/chemistry/*cytology/drug effects
MH  - Glomerulonephritis, Membranoproliferative/*chemically 
      induced/pathology/physiopathology
MH  - Growth Substances/genetics
MH  - Immunohistochemistry
MH  - In Vitro Techniques
MH  - Interleukin-1/genetics
MH  - Macrophages/cytology/drug effects
MH  - Male
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Wistar
MH  - Trimeresurus
MH  - Tumor Necrosis Factor-alpha/genetics
EDAT- 2002/10/10 04:00
MHDA- 2003/03/13 04:00
CRDT- 2002/10/10 04:00
PHST- 2002/10/10 04:00 [pubmed]
PHST- 2003/03/13 04:00 [medline]
PHST- 2002/10/10 04:00 [entrez]
AID - 64115 [pii]
AID - 10.1159/000064115 [doi]
PST - ppublish
SO  - Nephron. 2002;92(3):665-72. doi: 10.1159/000064115.