PMID- 12375327
OWN - NLM
STAT- MEDLINE
DCOM- 20030224
LR  - 20220409
IS  - 0315-162X (Print)
IS  - 0315-162X (Linking)
VI  - 29
IP  - 10
DP  - 2002 Oct
TI  - Clues to pathogenesis of spondyloarthropathy derived from synovial fluid 
      mononuclear cell gene expression profiles.
PG  - 2159-64
AB  - OBJECTIVE: To use gene expression profiles of spondyloarthropathy (SpA) synovial 
      fluid mononuclear cells (SFMC) to determine if there are transcripts that support 
      the unfolded protein response (UPR) hypothesis, and to identify which 
      cytokines/chemokines are being expressed and which cell fractions are involved. 
      METHODS: Gene expression profiles were generated by microarray screening of SFMC 
      of 5 patients with SpA, 5 patients with rheumatoid arthritis (RA), and peripheral 
      blood mononuclear cells (PBMC) of 6 controls. Results were validated by reverse 
      transcription polymerase chain reaction using samples from a larger panel of 
      subjects. RESULTS: The repertoires of proinflammatory cytokines/chemokines 
      expressed by SpA and RA SFMC were very similar: monocyte chemotractant protein 1 
      (MCP-1), interleukin 8 (IL-8), IL-1beta, endothelial-monocyte activating 
      polypeptide II, interferon-gamma, and tumor necrosis factor-alpha. MCP-1 was 
      highly expressed in SpA SFMC. There was enhanced expression of immunoglobulin 
      heavy chain binding protein (BiP) in SpA, which is compatible with the UPR 
      hypothesis. BiP was most highly expressed in the adherent fraction of SpA SFMC. 
      CONCLUSION: Previous data postulating UPR in SpA are based on in vitro 
      experiments with transfected cell lines. Our patient derived data suggest that it 
      also occurs in vivo in the macrophages of SpA joints.
FAU - Gu, Jieruo
AU  - Gu J
AD  - University of California Los Angeles, 90095, USA.
FAU - Rihl, Markus
AU  - Rihl M
FAU - Marker-Hermann, Elisabeth
AU  - Marker-Hermann E
FAU - Baeten, Dominique
AU  - Baeten D
FAU - Kuipers, Jens G
AU  - Kuipers JG
FAU - Song, Yeong Wook
AU  - Song YW
FAU - Maksymowych, Walter P
AU  - Maksymowych WP
FAU - Burgos-Vargas, Ruben
AU  - Burgos-Vargas R
FAU - Veys, Eric M
AU  - Veys EM
FAU - De Keyser, Filip
AU  - De Keyser F
FAU - Deister, Helmuth
AU  - Deister H
FAU - Xiong, Momiao
AU  - Xiong M
FAU - Huang, Feng
AU  - Huang F
FAU - Tsai, Wen Chan
AU  - Tsai WC
FAU - Yu, David Tak Yan
AU  - Yu DT
LA  - eng
GR  - GM56515/GM/NIGMS NIH HHS/United States
GR  - HL5448/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Canada
TA  - J Rheumatol
JT  - The Journal of rheumatology
JID - 7501984
RN  - 0 (Chemokines)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Chemokines/*genetics/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Gene Expression
MH  - Gene Expression Profiling
MH  - Genetic Testing
MH  - Humans
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - Middle Aged
MH  - *Oligonucleotide Array Sequence Analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spondylarthropathies/*genetics/metabolism/pathology
MH  - Synovial Fluid/cytology/*metabolism
EDAT- 2002/10/11 04:00
MHDA- 2003/02/25 04:00
CRDT- 2002/10/11 04:00
PHST- 2002/10/11 04:00 [pubmed]
PHST- 2003/02/25 04:00 [medline]
PHST- 2002/10/11 04:00 [entrez]
AID - 0315162X-29-2159 [pii]
PST - ppublish
SO  - J Rheumatol. 2002 Oct;29(10):2159-64.