PMID- 12377409
OWN - NLM
STAT- MEDLINE
DCOM- 20021105
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 137
IP  - 1
DP  - 2002 Aug
TI  - Synovial sarcoma with a secondary chromosome change der(22)t(17;22)(q12;q12).
PG  - 23-8
AB  - A consistent, pathognomonic translocation, most commonly a balanced reciprocal 
      translocation, t(X;18) (p11.2;q11.2), is found in more than 90% of synovial 
      sarcomas. We report here a secondary chromosome change, der(22)t(17;22)(q12;q12), 
      in addition to the primary t(X;18)(p11.2;q11.2) in a biphasic synovial sarcoma 
      that occurred in the thigh of a 34-year-old woman. Although the karyotype of the 
      primary tumor exhibited 46,X,t(X;18)(p11.2;q11.2), the recurrent tumor showed 
      46,X,der(X)t(X;18)(p11.2;q11.2),der(22) t(17;22)(q12;q12). The SYT-SSX1 fusion 
      transcript was demonstrated in the primary and recurrent tumors using a reverse 
      transcriptase polymerase chain reaction (RT-PCR). Southern blot analysis also 
      confirmed that the detected messages were derived from the SYT-SSX fusion gene. 
      However, we could not detect the EWS-E1AF fusion gene that has been reported to 
      be generated through a t(17;22)(q12;q12) by RT-PCR. Furthermore, fluorescence in 
      situ hybridization (FISH) with cosmid probes corresponding to loci flanking the 
      EWSR1 region demonstrated no split of chromosome 22 in all analyzed interphase 
      nuclei. To our knowledge, this is the first reported case of synovial sarcoma in 
      which an additional (secondary) chromosome change, der(22)t(17;22)(q12;q12), has 
      been demonstrated.
FAU - Nishio, Jun
AU  - Nishio J
AD  - Department of Pathology, School of Medicine, Fukuoka University, Fukuoka, Japan. 
      nishio@minf.med.fukuoka-u.ac.jp
FAU - Iwasaki, Hiroshi
AU  - Iwasaki H
FAU - Ishiguro, Masako
AU  - Ishiguro M
FAU - Ohjimi, Yuko
AU  - Ohjimi Y
FAU - Isayama, Teruto
AU  - Isayama T
FAU - Naito, Masatoshi
AU  - Naito M
FAU - Kaneko, Yasuhiko
AU  - Kaneko Y
FAU - Kamada, Nanao
AU  - Kamada N
FAU - Kikuchi, Masahiro
AU  - Kikuchi M
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - 0 (DNA Primers)
RN  - 0 (Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Repressor Proteins)
RN  - 0 (SS18 protein, human)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Chromosome Aberrations
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 17
MH  - *Chromosomes, Human, Pair 22
MH  - DNA Primers
MH  - Female
MH  - Humans
MH  - Karyotyping
MH  - Proteins/genetics
MH  - Proto-Oncogene Proteins
MH  - Repressor Proteins
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sarcoma, Synovial/*genetics/pathology
MH  - Transcription, Genetic
MH  - *Translocation, Genetic
EDAT- 2002/10/16 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/10/16 04:00
PHST- 2002/10/16 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/10/16 04:00 [entrez]
AID - S0165460802005502 [pii]
AID - 10.1016/s0165-4608(02)00550-2 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2002 Aug;137(1):23-8. doi: 10.1016/s0165-4608(02)00550-2.