PMID- 12377624
OWN - NLM
STAT- MEDLINE
DCOM- 20030113
LR  - 20060302
IS  - 1000-2588 (Print)
IS  - 1000-2588 (Linking)
VI  - 22
IP  - 10
DP  - 2002 Oct
TI  - Detection of soluble HLA class I antigens by ELISA and determination of its 
      reference value in population of Guangdong province.
PG  - 931-3
AB  - OBJECTIVE: To explore a method for quantitative detection of soluble human 
      leukocyte antigen class I(HLA-I) antigens, with the assistance of which we 
      attempt to define the reference value of the antigen in the population of 
      Guangdong Province. METHODS: Sandwich enzyme-linked immunosorbent assay (ELISA) 
      was employed with W6/32 monoclonal antibody serving as the solid phase antibody 
      and beta2 microglobulin antibody as the first antibody. HRP-labeled second 
      antibody and the substrate were added for enzyme-catalyzed coloring. On the basis 
      of the standard curve obtained by sHLA-I standard reagent in serial dilution, the 
      concentration of sHLA-I antigens in the samples of 60 normal subjects from the 
      population of Guangdong province was detected. RESULTS: The sensitivity of this 
      assay was 2.84 ng/ml with variation coefficients of 5.80% within the same batch 
      and 9.00% between batches. The recovery rate ranged from 98.57% to 100.15%. The 
      level of sHLA-I antigens in normal individuals was 699.54+/-360.10 ng/ml. 
      CONCLUSION: Sandwich ELISA is sensitive, specific and stable in the detection of 
      sHLA-I.
FAU - Wu, Tao
AU  - Wu T
AD  - Department of Transfusion, Nanfang Hospital, First Military Medical University, 
      Guangzhou 510515, China.
FAU - Lan, Jiong-Cai
AU  - Lan JC
FAU - Zhang, Hong
AU  - Zhang H
FAU - Wang, Cong-Rong
AU  - Wang CR
FAU - Mao, Gao-Cai
AU  - Mao GC
LA  - eng
PT  - Journal Article
PL  - China
TA  - Di Yi Jun Yi Da Xue Xue Bao
JT  - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
JID - 9426110
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Adult
MH  - China
MH  - Enzyme-Linked Immunosorbent Assay/methods/standards
MH  - Female
MH  - Histocompatibility Antigens Class I/*blood
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Solubility
EDAT- 2002/10/16 04:00
MHDA- 2003/01/14 04:00
CRDT- 2002/10/16 04:00
PHST- 2002/10/16 04:00 [pubmed]
PHST- 2003/01/14 04:00 [medline]
PHST- 2002/10/16 04:00 [entrez]
PST - ppublish
SO  - Di Yi Jun Yi Da Xue Xue Bao. 2002 Oct;22(10):931-3.