PMID- 12377629
OWN - NLM
STAT- MEDLINE
DCOM- 20030113
LR  - 20060302
IS  - 1000-2588 (Print)
IS  - 1000-2588 (Linking)
VI  - 22
IP  - 10
DP  - 2002 Oct
TI  - Preparation and identification of monoclonal antibodies against human 
      brain-derived neurotrophic factor.
PG  - 944-5
AB  - OBJECTIVE: To prepare monoclonal antibodies (mAbs) against human brain-derived 
      neurotrophic factor (BDNF). METHODS: BALB/C mice were immunized with purified 
      BDNF protein in conjunction with acid-treated Salmonella (antigen:thallus=1:5), 
      and mAbs were subsequently derived by hybridoma technique. RESULTS: Three 
      hybridoma cell lines secreting anti-BDNF mAbs were obtained, designated as B1, B2 
      and 4D1 respectively and all categorized into IgG1 subtype. The titers of the 
      mAbs in the ascitic fluid of the rats ranged from 1x10(6) to 1x10(5), and their 
      relative affinities were B2>B1>4D1. CONCLUSION: mAbs against BDNF are 
      successfully prepared, which can be instrumental for further study of the 
      expression and distribution of BDNF in vivo, and the detection of BDNF produced 
      by genetic engineering.
FAU - Su, Jin
AU  - Su J
AD  - Department of Respiratory Diseases, Nanfang Hospital, First Military Medical 
      University, Guangzhou 510515, China.
FAU - Zhang, Ya-Li
AU  - Zhang YL
FAU - You, Chang-Xuan
AU  - You CX
FAU - Wu, Jin-Ya
AU  - Wu JY
FAU - Che, Xiao-Yan
AU  - Che XY
FAU - Wang, Xiao-Ning
AU  - Wang XN
LA  - eng
PT  - Journal Article
PL  - China
TA  - Di Yi Jun Yi Da Xue Xue Bao
JT  - Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA
JID - 9426110
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*biosynthesis/immunology
MH  - Brain
MH  - Brain-Derived Neurotrophic Factor/*immunology
MH  - Humans
MH  - Hybridomas
MH  - Mice
MH  - Mice, Inbred BALB C
EDAT- 2002/10/16 04:00
MHDA- 2003/01/14 04:00
CRDT- 2002/10/16 04:00
PHST- 2002/10/16 04:00 [pubmed]
PHST- 2003/01/14 04:00 [medline]
PHST- 2002/10/16 04:00 [entrez]
PST - ppublish
SO  - Di Yi Jun Yi Da Xue Xue Bao. 2002 Oct;22(10):944-5.