PMID- 12377944
OWN - NLM
STAT- MEDLINE
DCOM- 20021115
LR  - 20181130
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 72
IP  - 4
DP  - 2002 Oct
TI  - Salmonella virulence factor SipB induces activation and release of IL-18 in human 
      dendritic cells.
PG  - 743-51
AB  - Interleukin-18 (IL-18) plays an important role in innate and acquired immunity, 
      in particular against intracellular pathogens. However, little is known about the 
      microbial factors that trigger IL-18 secretion by dendritic cells (DCs). To 
      determine the influence of bacterial virulence factors on the activation and 
      release of IL-18, we infected human monocyte-derived DCs with virulence mutants 
      of the facultative intracellular pathogen Salmonella typhimurium. Our results 
      show that infection by S. typhimurium causes caspase-1-dependent activation of 
      IL-18 and triggers the release of IL-18 in human DCs. The secretion of IL-18 by 
      the DCs was closely correlated with the ability of the S. typhimurium strains to 
      induce apoptosis. We demonstrate that activation and release of IL-18 are blocked 
      by mutations in the Salmonella sipB gene, which encodes a virulence factor that 
      activates caspase-1 to induce apoptosis. These findings indicate that the 
      activation and release of IL-18 induced by bacterial virulence factors may 
      represent one component of innate immunity against the intracellular bacteria.
FAU - Dreher, Donatus
AU  - Dreher D
AD  - Division of Pneumology, University Hospital of Geneva, Switzerland. 
      dreher@dim.hcuge.ch
FAU - Kok, Menno
AU  - Kok M
FAU - Obregon, Carolina
AU  - Obregon C
FAU - Kiama, Stephen G
AU  - Kiama SG
FAU - Gehr, Peter
AU  - Gehr P
FAU - Nicod, Laurent P
AU  - Nicod LP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Bacterial Proteins)
RN  - 0 (Interleukin-18)
RN  - 0 (Membrane Proteins)
RN  - 0 (invasion protein B, Salmonella typhimurium)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 3.4.22.36 (Caspase 1)
SB  - IM
MH  - Apoptosis
MH  - Bacterial Proteins/*immunology
MH  - Caspase 1/immunology
MH  - Dendritic Cells/*immunology/microbiology
MH  - Humans
MH  - Interferon-gamma/immunology
MH  - Interleukin-18/*metabolism
MH  - Membrane Proteins/*immunology
MH  - Salmonella typhimurium/genetics/*immunology/pathogenicity
MH  - Virulence
EDAT- 2002/10/16 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/10/16 04:00
PHST- 2002/10/16 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/10/16 04:00 [entrez]
PST - ppublish
SO  - J Leukoc Biol. 2002 Oct;72(4):743-51.