PMID- 12377948
OWN - NLM
STAT- MEDLINE
DCOM- 20021115
LR  - 20201219
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 72
IP  - 4
DP  - 2002 Oct
TI  - Role of the autocrine chemokines MIP-1alpha and MIP-1beta in the metastatic 
      behavior of murine T cell lymphoma.
PG  - 780-9
AB  - The ESb-MP T-cell line is a highly malignant murine lymphoma, which 
      preferentially metastasizes toward the kidney. This could be a result of the 
      local production of monocyte chemoattractant protein-1 (MCP-1) and regulated on 
      activation, normal T expressed and secreted (RANTES), which are chemotactic for 
      ESb-MP cells. Here, we demonstrate that ESb-MP cells are already responsive to 
      the chemotactic activity of macrophage inflammatory protein-1alpha (MIP-1alpha) 
      and MIP-1beta from 1 ng/ml onward. Moreover, upon stimulation with 
      lipopolysaccharide (LPS) or virus, ESb-MP cells themselves produce significant 
      amounts of MIP-1 ( approximately 200 ng/ml). Indeed, the major autocrine 
      chemoattractants, isolated from ESb-MP cells, were intact MIP-1alpha and 
      MIP-1beta. Pretreatment with LPS or addition of MIP-1 inhibited the in vitro 
      migration of ESb-MP cells toward various chemokines. Moreover, compared with 
      untreated lymphoma cells, LPS-treated cells produced significantly less 
      metastasis in mice. The results represented here suggest that the role of 
      chemokines in attracting tumor cells at secondary sites depends on a balance 
      between autocrine-produced and tissue-derived chemokines. This delicate balance 
      should be considered in the design of antichemokine strategies in different tumor 
      types.
FAU - Menten, Patricia
AU  - Menten P
AD  - Laboratory of Molecular Immunology, Rega Institute for Medical Research, Leuven, 
      Belgium.
FAU - Saccani, Alessandra
AU  - Saccani A
FAU - Dillen, Chris
AU  - Dillen C
FAU - Wuyts, Anja
AU  - Wuyts A
FAU - Struyf, Sofie
AU  - Struyf S
FAU - Proost, Paul
AU  - Proost P
FAU - Mantovani, Alberto
AU  - Mantovani A
FAU - Wang, Ji Ming
AU  - Wang JM
FAU - Van Damme, Jo
AU  - Van Damme J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Macrophage Inflammatory Proteins)
SB  - IM
MH  - Animals
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Disease Models, Animal
MH  - Lipopolysaccharides/pharmacology
MH  - Lymphoma, T-Cell/*physiopathology
MH  - Macrophage Inflammatory Proteins/*physiology
MH  - Mice
MH  - Mice, Inbred DBA
MH  - Neoplasm Metastasis
MH  - Neoplasms, Experimental
MH  - Tumor Cells, Cultured
EDAT- 2002/10/16 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/10/16 04:00
PHST- 2002/10/16 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/10/16 04:00 [entrez]
PST - ppublish
SO  - J Leukoc Biol. 2002 Oct;72(4):780-9.