PMID- 12379176
OWN - NLM
STAT- MEDLINE
DCOM- 20030429
LR  - 20190922
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 32
IP  - 4
DP  - 2002 Oct
TI  - Characterization of Ca(2+) signaling pathways in human mesenchymal stem cells.
PG  - 165-74
AB  - Human mesenchymal stem cells (HMSC) have the potential to differentiate into many 
      cell types. The physiological properties of HMSCs including their Ca(2+) 
      signaling pathways, however, are not well understood. We investigated Ca(2+) 
      influx and release functions in HMSCs. In Ca(2+) imaging experiments, spontaneous 
      Ca(2+) oscillations were observed in 36 of 50 HMSCs. The Ca(2+) oscillations were 
      completely blocked by the application of 10 micro M cyclopiazonic acid (CPA) or 1 
      micro M thapsigargin (TG). A brief application of 1 micro M acetylcholine (ACh) 
      induced a transient increase of [Ca(2+)](i) but the application of caffeine (10 
      mM) did not induce any Ca(2+) transient. When the stores were depleted with 
      Ca(2+)-ATPase blockers (CPA or TG) or muscarinic agonists (ACh), store-operated 
      Ca(2+) (SOC) entry was observed. Using the patch-clamp technique, store-operated 
      Ca(2+) currents (I(SOC)) could be recorded in cells treated with ACh or CPA, but 
      voltage-operated Ca(2+) currents (VOCCs) were not elicited in most of the cells 
      (17/20), but in 15% of cells examined, small dihydropyridine (DHP)-sensitive 
      Ca(2+) currents were recorded. Using RT-PCR, mRNAs were detected for inositol 
      1,4,5-trisphosphate receptor (InsP(3)R) type I, II, and III and DHP receptors 
      alpha1A and alpha1H were detected, but mRNA was not detected for ryanodine 
      receptor (RyR) or N-type Ca(2+) channels. These results suggest that in 
      undifferentiated HMSCs, Ca(2+) release is mediated by InsP(3)Rs and Ca(2+) entry 
      through plasma membrane is mainly mediated by the SOCs channels with a little 
      contribution of VOCCs.
FAU - Kawano, S
AU  - Kawano S
AD  - Department of Cardiovascular Diseases, Medical Research Institute, Tokyo Medical 
      and Dental University, Tokyo, Japan. seiko-kawano@tmd.ac.jp
FAU - Shoji, S
AU  - Shoji S
FAU - Ichinose, S
AU  - Ichinose S
FAU - Yamagata, K
AU  - Yamagata K
FAU - Tagami, M
AU  - Tagami M
FAU - Hiraoka, M
AU  - Hiraoka M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Calcium Channels)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*metabolism
MH  - Calcium Channels/metabolism
MH  - *Calcium Signaling
MH  - Cell Membrane/metabolism
MH  - Cell Nucleus/ultrastructure
MH  - Endoplasmic Reticulum/ultrastructure
MH  - Humans
MH  - Mesoderm/cytology/*metabolism/ultrastructure
MH  - Patch-Clamp Techniques
MH  - Stem Cells/cytology/*metabolism/ultrastructure
MH  - Transport Vesicles/ultrastructure
EDAT- 2002/10/16 04:00
MHDA- 2003/04/30 05:00
CRDT- 2002/10/16 04:00
PHST- 2002/10/16 04:00 [pubmed]
PHST- 2003/04/30 05:00 [medline]
PHST- 2002/10/16 04:00 [entrez]
AID - S0143416002001240 [pii]
AID - 10.1016/s0143416002001240 [doi]
PST - ppublish
SO  - Cell Calcium. 2002 Oct;32(4):165-74. doi: 10.1016/s0143416002001240.