PMID- 12379333
OWN - NLM
STAT- MEDLINE
DCOM- 20021213
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 270
IP  - 1
DP  - 2002 Dec 1
TI  - Development of an assay for quantification of linkage-specific O-acetylated 
      sialoglycans on erythrocytes; its application in Indian visceral leishmaniasis.
PG  - 1-10
AB  - We have developed a noninvasive approach for the quantification of 
      linkage-specific 9-O-acetylated sialoglycans on mammalian erythrocytes using a 
      lectin, Achatinin-H, whose lectinogenic epitope has previously been defined as 
      9-O-acetylated sialoglycoconjugates (9-O-AcSGs) alpha 2-->6 linked to subterminal 
      GalNAc. Titration and checkerboard analysis were performed to optimize the assay 
      using rabbit, rat and human erythrocytes that contain differing amounts of this 
      glycotope. Assay specificity was established by decreased binding of erythrocytes 
      to immobilised Achatinin-H when pre-incubated with excess lectin. The intra-assay 
      coefficient of variation (CV) for rat and human erythrocytes was 8.6-9.2% and 
      11.1-13.0%, respectively. The inter-assay CV for rat and human erythrocytes was 
      9.9-10.1% and 15.2-16.6%, respectively. In previous studies, we have identified 
      an enhanced presence of cell surface 9-O-AcSGs on the erythrocytes of patients 
      with visceral leishmaniasis (VL) [Am. J. Trop. Med. Hyg. 58 (1998) 551]. Our 
      assay when evaluated on erythrocytes from VL patients (n=30) showed a fourfold 
      increase in lectin binding as compared to endemic controls. The mean +/- S.E.M. 
      of the A(405) nm value was 1.14 +/- 0.04 vs. 0.23 +/- 0.03, respectively 
      (p<0.0001). Following effective chemotherapy, a significant reduction of this 
      glycotope on the erythrocytes of VL patients indicates that this assay has both a 
      diagnostic and prognostic potential. Taken together, we conclude that this 
      antigen-based assay is a specific and reproducible method for monitoring the 
      disease status of VL patients and could be used in retrospective and prospective 
      trials.
FAU - Chava, Anil Kumar
AU  - Chava AK
AD  - Immunobiology Division, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick 
      Road, Jadavpur, Calcutta 700 032, India.
FAU - Chatterjee, Mitali
AU  - Chatterjee M
FAU - Sundar, Shyam
AU  - Sundar S
FAU - Mandal, Chitra
AU  - Mandal C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Glycoconjugates)
RN  - 0 (Lectins)
RN  - 0 (Polysaccharides)
RN  - 0 (achatinin(H))
RN  - GZP2782OP0 (N-Acetylneuraminic Acid)
SB  - IM
MH  - Acetylation
MH  - Adolescent
MH  - Adult
MH  - Animals
MH  - Child
MH  - Erythrocytes/*chemistry
MH  - Female
MH  - Glycoconjugates/*analysis
MH  - Humans
MH  - India
MH  - Lectins/analysis
MH  - Leishmaniasis, Visceral/*blood/diagnosis
MH  - Male
MH  - Middle Aged
MH  - N-Acetylneuraminic Acid/*analysis
MH  - Polysaccharides/*analysis
MH  - Rabbits
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2002/10/16 04:00
MHDA- 2002/12/17 04:00
CRDT- 2002/10/16 04:00
PHST- 2002/10/16 04:00 [pubmed]
PHST- 2002/12/17 04:00 [medline]
PHST- 2002/10/16 04:00 [entrez]
AID - S0022175902002168 [pii]
AID - 10.1016/s0022-1759(02)00216-8 [doi]
PST - ppublish
SO  - J Immunol Methods. 2002 Dec 1;270(1):1-10. doi: 10.1016/s0022-1759(02)00216-8.