PMID- 12379773
OWN - NLM
STAT- MEDLINE
DCOM- 20021119
LR  - 20230216
IS  - 0023-6837 (Print)
IS  - 0023-6837 (Linking)
VI  - 82
IP  - 10
DP  - 2002 Oct
TI  - Cigarette smoke induces rapid changes in gene expression in pulmonary arteries.
PG  - 1391-8
AB  - The pathogenesis of cigarette smoke-induced pulmonary hypertension is not well 
      characterized. We used RT-PCR to examine gene expression of nitric oxide synthase 
      2 (NOS-2), nitric oxide synthase 3 (NOS-3), endothelin, and vascular endothelial 
      growth factor (VEGF) and its flk-1 receptor (VEGF-R) in main pulmonary arteries 
      and in intraparenchymal arteries microdissected from alcohol-fixed paraffin 
      blocks. The main pulmonary artery and intraparenchymal vessels responded in a 
      similar fashion, with up-regulation of endothelin, VEGF, and VEGF-R gene 
      expression evident by 2 hours after smoke exposure. Up-regulation of gene 
      expression was still present at 24 hours after exposure, and at this time there 
      was also a small increase in NOS-2. As a comparison, we examined the trachea and 
      microdissected intraparenchymal airways and found up-regulation of endothelin and 
      NOS-2 at 2 hours and additional up-regulation of NOS-3 at 24 hours. These 
      findings suggest that the pulmonary vasculature very rapidly responds to 
      cigarette smoke with up-regulation of mediators that control vascular cell 
      proliferation and vascular constriction. These changes support the idea that 
      pulmonary hypertension in cigarette smokers reflects a direct effect of smoke on 
      the vasculature. The pattern of response in the vessels is distinctly different 
      from that in the airways.
FAU - Wright, Joanne L
AU  - Wright JL
AD  - Department of Pathology, University of British Columbia, Vancouver, British 
      Columbia, Canada. jlwright@interchange.ubc.ca
FAU - Tai, Hsin
AU  - Tai H
FAU - Dai, Jin
AU  - Dai J
FAU - Churg, Andrew
AU  - Churg A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Lab Invest
JT  - Laboratory investigation; a journal of technical methods and pathology
JID - 0376617
RN  - 0 (Smoke)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - *Gene Expression Regulation
MH  - Hypertension, Pulmonary/*etiology
MH  - Muscle, Smooth, Vascular/pathology
MH  - Pulmonary Artery/*pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Smoke/*adverse effects
MH  - Smoking/*adverse effects
EDAT- 2002/10/16 04:00
MHDA- 2002/11/26 04:00
CRDT- 2002/10/16 04:00
PHST- 2002/10/16 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/10/16 04:00 [entrez]
AID - S0023-6837(22)03650-9 [pii]
AID - 10.1097/01.lab.0000032806.45023.08 [doi]
PST - ppublish
SO  - Lab Invest. 2002 Oct;82(10):1391-8. doi: 10.1097/01.lab.0000032806.45023.08.