PMID- 12380672
OWN - NLM
STAT- MEDLINE
DCOM- 20030130
LR  - 20160803
IS  - 1476-7058 (Print)
IS  - 1476-4954 (Linking)
VI  - 11
IP  - 3
DP  - 2002 Mar
TI  - Maternal intravascular inflammation in preterm premature rupture of membranes.
PG  - 171-5
AB  - OBJECTIVE: Intrauterine inflammation has been implicated in the mechanisms 
      responsible for preterm premature rupture of membranes (PROM). However, it is 
      unclear whether this inflammatory process remains localized to the uterus, at the 
      site of membrane rupture, or extends to the maternal compartment. Flow cytometric 
      analysis is a sensitive method to assess the presence and magnitude of in vivo 
      inflammation. This study was conducted to determine whether preterm PROM is 
      associated with changes in the phenotypic and metabolic characteristics of 
      maternal granulocytes and monocytes consistent with the presence of maternal 
      intravascular inflammation. STUDY DESIGN: A prospective cross-sectional study was 
      performed including patients with preterm PROM (n = 43) and normal pregnancy (n = 
      51). Maternal intravascular inflammation was studied using flow cytometry. 
      Maternal blood was assayed to determine granulocyte and monocyte phenotype using 
      monoclonal antibodies, which included cluster differentiation (CD) markers CD11b, 
      CD14, CD15, CD16, CD18, CD49d, CD62L, CD64, CD66b and human leukocyte antigen 
      (HLA)-DR. The quantities of basal intracellular reactive oxygen species (iROS) 
      and oxidative burst was assessed. Statistical analysis was conducted with the use 
      of non-parametric methods. A p value < 0.01 was considered significant. RESULTS: 
      Preterm PROM was associated with a significant increase in the median mean 
      channel brightness (MCB) of CD11b, CD14, CD64 and CD66b on granulocytes and 
      median MCB of CD11b on monocytes. The oxidative burst and the stimulation index 
      in both cell types were higher in preterm PROM than in normal pregnancy (p < 
      0.01). CONCLUSION: Preterm PROM is associated with phenotypic and metabolic 
      changes in circulating granulocytes and monocytes.
FAU - Gervasi, M T
AU  - Gervasi MT
AD  - Perinatology Research Branch, National Institute of Child Health and Human 
      Development, Bethesda, Maryland, USA.
FAU - Chaiworapongsa, T
AU  - Chaiworapongsa T
FAU - Naccasha, N
AU  - Naccasha N
FAU - Pacora, P
AU  - Pacora P
FAU - Berman, S
AU  - Berman S
FAU - Maymon, E
AU  - Maymon E
FAU - Kim, J C
AU  - Kim JC
FAU - Kim, Y M
AU  - Kim YM
FAU - Yoshimatsu, J
AU  - Yoshimatsu J
FAU - Espinoza, J
AU  - Espinoza J
FAU - Romero, R
AU  - Romero R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Matern Fetal Neonatal Med
JT  - The journal of maternal-fetal & neonatal medicine : the official journal of the 
      European Association of Perinatal Medicine, the Federation of Asia and Oceania 
      Perinatal Societies, the International Society of Perinatal Obstetricians
JID - 101136916
RN  - 0 (Biomarkers)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Biomarkers/blood
MH  - Cross-Sectional Studies
MH  - Female
MH  - Fetal Membranes, Premature Rupture/*immunology
MH  - Flow Cytometry
MH  - Granulocytes/*immunology
MH  - HLA-DR Antigens/immunology
MH  - Humans
MH  - Inflammation/blood/immunology
MH  - Monocytes/*immunology
MH  - Pregnancy
MH  - Prospective Studies
MH  - Reactive Oxygen Species/immunology
MH  - Respiratory Burst/immunology/physiology
MH  - Uterine Diseases/blood/*immunology
MH  - Uterus/microbiology
EDAT- 2002/10/17 04:00
MHDA- 2003/01/31 04:00
CRDT- 2002/10/17 04:00
PHST- 2002/10/17 04:00 [pubmed]
PHST- 2003/01/31 04:00 [medline]
PHST- 2002/10/17 04:00 [entrez]
AID - 10.1080/jmf.11.3.171.175 [doi]
PST - ppublish
SO  - J Matern Fetal Neonatal Med. 2002 Mar;11(3):171-5. doi: 10.1080/jmf.11.3.171.175.