PMID- 12381092
OWN - NLM
STAT- MEDLINE
DCOM- 20030605
LR  - 20190819
IS  - 1346-9843 (Print)
IS  - 1346-9843 (Linking)
VI  - 66
IP  - 10
DP  - 2002 Oct
TI  - Cloning and functional expression of a novel splice variant of rat TRPC4.
PG  - 954-8
AB  - Transient receptor potential protein 4 (TRPC4) has been identified as a candidate 
      for the capacitative calcium entry (CCE) channels, but its functional role is 
      still controversial. Using a RT-PCR technique, a novel isoform of TRPC4, 
      designated rTRPC4gamma, was isolated. It was nearly identical to full-length 
      rTRPC4 (rTRPC4alpha), except that it lacked 53 nucleotides that correspond to the 
      predicted linker between the second and third transmembrane domain of 
      rTRPC4alpha, and its mRNA was expressed in brain and heart. This splice variant 
      encoded a potential protein of 400 residues that consists of an amino-terminal 
      cytoplasmic domain and 2 transmembrane domains by a frameshift mutation. When 
      rTRPC4gamma cDNA was transiently transfected to HEK-293 cells, thapsigargin 
      (TG)-induced Ca2+ entry was suppressed significantly. By contrast, expression of 
      rTRPC4 a did not affect TG-induced Ca2+ entry. To investigate the subcellular 
      localization, plasmids were constructed with green fluorescence protein (GFP) as 
      an amino-terminal fusion to rTRPC4 variants. GFP-rTRPC4gamma fusion protein, 
      unlike GFP-rTRPC4alpha, was localized to the cytoplasm as well as plasma 
      membrane. These results suggest that rTRPC4gamma may play a modulatory role in 
      CCE channel activity in the brain and heart.
FAU - Satoh, Eisaku
AU  - Satoh E
AD  - Department of Pharmacology, Akita University School of Medicine, Japan.
FAU - Ono, Kyoichi
AU  - Ono K
FAU - Xu, Feng
AU  - Xu F
FAU - Iijima, Toshihiko
AU  - Iijima T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Circ J
JT  - Circulation journal : official journal of the Japanese Circulation Society
JID - 101137683
RN  - 0 (Calcium Channels)
RN  - 0 (DNA, Complementary)
RN  - 0 (Protein Isoforms)
RN  - 0 (TRPC Cation Channels)
RN  - 67526-95-8 (Thapsigargin)
RN  - 8Y164V895Y (Carbachol)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Alternative Splicing/*genetics
MH  - Animals
MH  - Base Sequence
MH  - Calcium/metabolism/pharmacology
MH  - Calcium Channels/*genetics/metabolism/*physiology
MH  - Carbachol/pharmacology
MH  - Cell Line
MH  - *Cloning, Molecular
MH  - DNA, Complementary/isolation & purification
MH  - Genetic Variation
MH  - Humans
MH  - Microscopy, Confocal
MH  - Molecular Sequence Data
MH  - Protein Isoforms/genetics
MH  - Rats
MH  - TRPC Cation Channels
MH  - Thapsigargin/pharmacology
MH  - Transfection
EDAT- 2002/10/17 04:00
MHDA- 2003/06/06 05:00
CRDT- 2002/10/17 04:00
PHST- 2002/10/17 04:00 [pubmed]
PHST- 2003/06/06 05:00 [medline]
PHST- 2002/10/17 04:00 [entrez]
AID - 10.1253/circj.66.954 [doi]
PST - ppublish
SO  - Circ J. 2002 Oct;66(10):954-8. doi: 10.1253/circj.66.954.