PMID- 12382674
OWN - NLM
STAT- MEDLINE
DCOM- 20030418
LR  - 20121115
IS  - 0963-6897 (Print)
IS  - 0963-6897 (Linking)
VI  - 11
IP  - 5
DP  - 2002
TI  - Implantation of BDNF-producing packaging cells into brain.
PG  - 459-64
AB  - In order to invent a screening system to check in vivo gene function and the 
      efficiency of gene transfer mediated by a retroviral vector system, we 
      established a novel packaging cell, PacC6/A8, that is transplantable to rat 
      brains. The packaging cell is based on the gene of the neuropatogenic retrovirus, 
      A8-V. For expression in the brain, a vector that expresses brain-derived 
      neurotrophic factor (BDNF) tagged by c-Myc-His6 (LxA/bdmh) was constructed. After 
      transfection of LxA/bdmh to PacC6/A8, a cloned cell line, PacC6/A8/bmh, was 
      established. PacC6/A8/bmh cells stably produced pseudotyped retroviruses carrying 
      LxA/bdmh. For a control, a retroviral vector that bears the gene that codes 
      enhanced green fluorescent protein (EGFP) tagged by C-Mic-His6 was also created 
      and used for the establishment of PacC6/A8/gfmh cells that produce pseudotyped 
      retroviruses carrying LxA/gfmh. PacC6/A8/bmh and PacC6/A8/gfmh cells were 
      injected to the brain of newborn rats. A tumor was formed in all the rats 
      injected that did not exhibit any symptoms until 3-4 weeks after the injection. A 
      histological study of the injected rats revealed that the transferred BDNF gene 
      was expressed in the brain of rats injected with PacC6/A8/bmh cells, but not in 
      rats with PacC6/A8/gfmh cells. Interestingly, many activated microglia had 
      migrated into the tumor induced by PacC6/A8/bmh cells, and expressed a high 
      amount of BDNF.
FAU - Fukumitsu, Hidefumi
AU  - Fukumitsu H
AD  - Institute of Life Science, Soka University, Hachioji, Tokyo, Japan.
FAU - Takase-Yoden, Sayaka
AU  - Takase-Yoden S
FAU - Furukawa, Shoei
AU  - Furukawa S
FAU - Nemoto, Kiyomitsu
AU  - Nemoto K
FAU - Ikeda, Tomio
AU  - Ikeda T
FAU - Watanabe, Rihito
AU  - Watanabe R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Transplant
JT  - Cell transplantation
JID - 9208854
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Luminescent Proteins)
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Brain/anatomy & histology/*surgery
MH  - Brain-Derived Neurotrophic Factor/biosynthesis/*genetics
MH  - COS Cells
MH  - Cell Transplantation/*methods
MH  - Genetic Therapy/methods
MH  - *Genetic Vectors/administration & dosage
MH  - Green Fluorescent Proteins
MH  - Immunohistochemistry
MH  - Luminescent Proteins
MH  - Rats
MH  - Retroviridae/*genetics/isolation & purification
MH  - Transduction, Genetic/methods
MH  - Tumor Cells, Cultured
MH  - Virus Assembly
EDAT- 2002/10/18 04:00
MHDA- 2003/04/19 05:00
CRDT- 2002/10/18 04:00
PHST- 2002/10/18 04:00 [pubmed]
PHST- 2003/04/19 05:00 [medline]
PHST- 2002/10/18 04:00 [entrez]
PST - ppublish
SO  - Cell Transplant. 2002;11(5):459-64.