PMID- 12383204
OWN - NLM
STAT- MEDLINE
DCOM- 20021118
LR  - 20190513
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 107
IP  - 2
DP  - 2002 Oct
TI  - Studies on the association between immunoglobulin E autoreactivity and 
      immunoglobulin E-dependent histamine-releasing factors.
PG  - 243-51
AB  - It has been reported that serum immunoglobulin E (IgE) from certain atopic 
      patients can sensitize basophils to release histamine in response to 
      IgE-dependent histamine-releasing factors (HRFs). It has also been shown that 
      patients suffering from severe forms of atopy may contain IgE autoantibodies. It 
      was investigated whether HRF-responsive sera contained IgE autoantibodies and if 
      there was an association between IgE autoreactivity and IgE-dependent 
      responsiveness to HRF. The presence of HRF-responsive IgE (IgE+) in serum of 
      patients with respiratory atopy was determined by stimulating stripped human 
      basophils sensitized by serum with peripheral blood mononuclear cell 
      (PBMC)-derived HRF, and measuring the release of histamine. In parallel, these 
      sera were screened for the presence of IgE autoantibodies to 
      nitrocellulose-blotted human cellular extracts. The capacity of IgE 
      autoantigen-containing preparations to induce histamine release was tested in the 
      stripped basophil assay. Eleven out of 52 sera contained IgE autoantibodies to 
      blotted cellular extracts of human PBMCs or of the human epithelial cell line 
      A431. No significant association was found between IgE autoreactivity and 
      IgE-dependent responsiveness to HRF: 7/26 IgE+ sera contained IgE to human 
      cellular extracts, and 4/26 of the sera without IgE+ did also. IgE 
      autoantigen-containing extracts did not induce histamine release of appropriately 
      sensitized basophils. By size-exclusion chromatography it was shown that a 32 000 
      MW autoantigen eluted in the >55 000 MW fraction, which indicates that this 
      protein forms polymers or complexes with other macromolecules. This might explain 
      the discrepancy between binding and histamine-releasing activity. A 20 000 MW 
      IgE-defined autoantigen cross-reacted with a shrimp allergen. Our results 
      indicate that IgE-reactivity to immunoblotted human protein and IgE-dependent HRF 
      activity are distinct entities that may co-occur in atopic patients.
FAU - Budde, Ilona Kleine
AU  - Budde IK
AD  - Department of Immunopathology, Sanquin Research at CLB, Landsteiner Laboratory, 
      Academic Medical Centre, University of Amsterdam, Plesmanlaan 125, Amsterdam, the 
      Netherlands.
FAU - de Heer, Pleuni G
AU  - de Heer PG
FAU - Natter, Susanne
AU  - Natter S
FAU - Mahler, Vera
AU  - Mahler V
FAU - van der Zee, Jaring S
AU  - van der Zee JS
FAU - Valenta, Rudolf
AU  - Valenta R
FAU - Aalberse, Rob C
AU  - Aalberse RC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Autoantigens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Antibodies, Anti-Idiotypic/*immunology
MH  - Asthma/immunology
MH  - Autoantigens/immunology
MH  - Basophils/immunology
MH  - Case-Control Studies
MH  - HeLa Cells
MH  - Histamine Release/*immunology
MH  - Humans
MH  - Hypersensitivity, Immediate/*immunology
MH  - Immunoglobulin E/*immunology
PMC - PMC1782795
EDAT- 2002/10/18 04:00
MHDA- 2002/11/26 04:00
PMCR- 2003/10/01
CRDT- 2002/10/18 04:00
PHST- 2002/10/18 04:00 [pubmed]
PHST- 2002/11/26 04:00 [medline]
PHST- 2002/10/18 04:00 [entrez]
PHST- 2003/10/01 00:00 [pmc-release]
AID - 1475 [pii]
AID - 10.1046/j.1365-2567.2002.01475.x [doi]
PST - ppublish
SO  - Immunology. 2002 Oct;107(2):243-51. doi: 10.1046/j.1365-2567.2002.01475.x.