PMID- 12383512
OWN - NLM
STAT- MEDLINE
DCOM- 20030516
LR  - 20190708
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 296
IP  - 1-2
DP  - 2002 Aug 21
TI  - Characterization of the promoter controlling Mona/Gads expression in the 
      megakaryocytic lineage.
PG  - 151-9
AB  - Mona/grb2 related adapter downstream of shc is a molecular adapter expressed in 
      platelets, T lymphocytes and myelomonocytic cells. Using human hematopoietic cell 
      lines, we have previously shown that lineage-specific Mona expression is achieved 
      through the production of two transcripts (named 1A and 1B) differing by their 5' 
      untranslated region (5'UTR). Thus, platelets and megakaryocytic cell lines K562 
      and HEL (Human Erythro-Leukemia) specifically express 1B messenger RNA (mRNA). We 
      report here characterization of the (-2031/+72) genomic region relative to the 
      putative transcription start site of 1B mRNA. We show this region is sufficient 
      to ensure specific reporter gene expression in megakaryocytic cell lines, and 
      that most promoter activity is contained in the (-225/+72) fragment. 
      Electro-mobility shift assay and mutational analyses indicated that GATA-1 and a 
      yet unidentified E-26 family member transcription factor are required for 1B 
      (-2031/+72) promoter activity. Thus, Mona 1B promoter exhibits typical features 
      of megakaryocyte-specific promoters.
FAU - Guyot, Boris
AU  - Guyot B
AD  - Centre de Genetique Moleculaire et Cellulaire, UMR CNRS 5534, Universite Claude 
      Bernard Lyon-1, 43, boulevard du 11 novembre 1918, Batiment Gregor Mendel, 16 rue 
      Raphael Dubois, 69622 Villeurbanne Cedex, France.
FAU - Mouchiroud, Guy
AU  - Mouchiroud G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Carrier Proteins)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Erythroid-Specific DNA-Binding Factors)
RN  - 0 (GATA1 Transcription Factor)
RN  - 0 (GATA1 protein, human)
RN  - 0 (GRAP2 protein, human)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-ets)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 1.13.12.- (Luciferases)
RN  - EC 3.2.1.23 (beta-Galactosidase)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - *Adaptor Proteins, Signal Transducing
MH  - Base Sequence
MH  - Binding Sites/genetics
MH  - Carrier Proteins/*genetics
MH  - Cell Differentiation/genetics
MH  - DNA-Binding Proteins/metabolism
MH  - Electrophoretic Mobility Shift Assay
MH  - Erythroid-Specific DNA-Binding Factors
MH  - GATA1 Transcription Factor
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Jurkat Cells
MH  - K562 Cells
MH  - Luciferases/genetics/metabolism
MH  - Megakaryocytes/*metabolism/pathology
MH  - Molecular Sequence Data
MH  - Promoter Regions, Genetic/*genetics
MH  - Protein Binding
MH  - Proto-Oncogene Proteins/metabolism
MH  - Proto-Oncogene Proteins c-ets
MH  - RNA, Messenger/genetics/metabolism
MH  - Recombinant Fusion Proteins/genetics/metabolism
MH  - Regulatory Sequences, Nucleic Acid/genetics
MH  - Sequence Deletion
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Transcription Factors/metabolism
MH  - Transfection
MH  - Tumor Cells, Cultured
MH  - U937 Cells
MH  - beta-Galactosidase/genetics/metabolism
EDAT- 2002/10/18 04:00
MHDA- 2003/05/17 05:00
CRDT- 2002/10/18 04:00
PHST- 2002/10/18 04:00 [pubmed]
PHST- 2003/05/17 05:00 [medline]
PHST- 2002/10/18 04:00 [entrez]
AID - S0378111902008442 [pii]
AID - 10.1016/s0378-1119(02)00844-2 [doi]
PST - ppublish
SO  - Gene. 2002 Aug 21;296(1-2):151-9. doi: 10.1016/s0378-1119(02)00844-2.