PMID- 12383643
OWN - NLM
STAT- MEDLINE
DCOM- 20030124
LR  - 20190901
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 89
IP  - 3-4
DP  - 2002 Oct 28
TI  - Increase in apoptotic polymorphonuclear neutrophils in peripheral blood after 
      intramammary infusion of Escherichia coli lipopolysaccharide.
PG  - 115-25
AB  - A transient increase in apoptotic polymorphonuclear neutrophils (PMNs) as 
      revealed by the terminal deoxynucleotidyl, transferase-mediated dUTP nick end 
      labeling (TUNEL) technique in bovine jugular and milk vein blood was observed 4 h 
      after intramammary infusion of Escherichia coli lipopolysaccharide (LPS) (jugular 
      vein; before infusion 10.1%, 4h 58.3%: milk vein; before infusion 13.2%, 4 h 
      76.6%) decrease in PMA-induced oxidative bursts of PMNs was also observed during 
      the same period and continued until 8 h after the infusion. TUNEL-positive cells 
      showed an intention of a Comet tail as detected by a single-cell gel 
      electrophoresis assay (Comet assay) and the morphological apoptotic future, 
      though DNA fragmentation was not clearly detected. A definite decrease in 
      peripheral PMNs and a marked increase in PMNs in the LPS-infused teat cistern 
      were observed during the same period. The migration of milk vein blood-derived 
      PMN and the expression of adhesion receptors (L-selectin and CD18) on PMN were 
      suppressed, accompanied by an increase in apoptotic cells. TUNEL-positive PMN 
      observed in normal animals showed a reduced migration capacity. The increase in 
      apoptotic PMNs observed in the LPS-infused cattle was thought to be due to the 
      remaining intravenous spontaneous apoptotic cells existing under the normal 
      condition (the aging cell), and this increase appeared to lower the expression of 
      adhesion receptors and the migration capacity. Decreased PMA-induced oxidative 
      burst activity in PMN was thought to be derived from these aging cells and 
      immature band cells appearing in the circulation as a subsequent event of 
      leukopenia and/or severe stress associated with mastitis. The results from the 
      present study indicate the possibility that the function of PMN in the 
      circulation at early stages of bovine mastitis is regulated by the kinetics of 
      PMN aging.
CI  - Copyright 2002 Elsevier Science B.V.
FAU - Yagi, Yukio
AU  - Yagi Y
AD  - Hokkaido Research Station, National Institute of Animal Health, 4 Hitsuigaoka, 
      Toyohira, Sapporo, Hokkaido 062-0045, Japan. yyagi@affrc.go.jp
FAU - Shiono, Hiroki
AU  - Shiono H
FAU - Shibahara, Tomoyuki
AU  - Shibahara T
FAU - Chikayama, Yukio
AU  - Chikayama Y
FAU - Nakamura, Ichiro
AU  - Nakamura I
FAU - Ohnuma, Aiko
AU  - Ohnuma A
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PT  - Randomized Controlled Trial
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Endotoxins)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Cattle/*immunology/microbiology
MH  - Cell Adhesion
MH  - Chemotaxis, Leukocyte
MH  - Comet Assay
MH  - Endotoxins/analysis/blood
MH  - Escherichia coli/*immunology
MH  - Female
MH  - In Situ Nick-End Labeling
MH  - Inflammation/immunology
MH  - Lipopolysaccharides/*administration & dosage/*immunology
MH  - Mammary Glands, Animal/immunology
MH  - Mastitis, Bovine/immunology
MH  - Milk/immunology
MH  - Neutrophils/*cytology/*immunology
MH  - Respiratory Burst
MH  - Time Factors
EDAT- 2002/10/18 04:00
MHDA- 2003/01/25 04:00
CRDT- 2002/10/18 04:00
PHST- 2002/10/18 04:00 [pubmed]
PHST- 2003/01/25 04:00 [medline]
PHST- 2002/10/18 04:00 [entrez]
AID - S016524270200199X [pii]
AID - 10.1016/s0165-2427(02)00199-x [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2002 Oct 28;89(3-4):115-25. doi: 
      10.1016/s0165-2427(02)00199-x.