PMID- 12383938
OWN - NLM
STAT- MEDLINE
DCOM- 20021209
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1573
IP  - 1
DP  - 2002 Oct 10
TI  - 2-Benzyloxybenzaldehyde inhibits formyl-methionyl-leucyl-phenylalanine 
      stimulation of phospholipase D activation in rat neutrophils.
PG  - 26-32
AB  - 2-Benzyloxybenzaldehyde (CCY1a) inhibited the 
      formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated phospholipase D 
      (PLD)-mediated products, phosphatidic acid (PA) and phosphatidylethanol (PEt) 
      formation in rat neutrophils in a concentration-dependent manner with IC(50) 
      values of 15.8+/-2.5 and 13.9+/-2.0 microM, respectively. The underlying cellular 
      signaling mechanism of CCY1a inhibition was investigated. CCY1a inhibited the 
      plateau phase but not the initial Ca(2+) spike of fMLP-stimulated Ca(2+) signal. 
      CCY1a did not inhibit the [Ca(2+)](i) change in Ca(2+)-free medium in response to 
      fMLP, but inhibited the [Ca(2+)](i) change by the subsequent addition of Ca(2+). 
      In addition, CCY1a treatment attenuated the fMLP-induced protein tyrosine 
      phosphorylation. The membrane translocation of ADP-ribosylation factor (ARF) and 
      Rho A proteins in neutrophils stimulated with fMLP was attenuated by CCY1a in a 
      concentration-dependent manner. In a cell-free system, neither the membrane 
      association of ARF and Rho A caused by GTPgammaS nor the phorbol myristate 
      acetate-stimulated membrane translocation of Rho A was suppressed significantly 
      by CCY1a. These results indicate that the attenuation of protein tyrosine 
      phosphorylation, blockade of Ca(2+) entry, and the suppression of ARF and Rho A 
      membrane translocation are probably obligatory for the CCY1a inhibition of PLD 
      activity in rat neutrophils in response to fMLP.
FAU - Wang, Jih-Pyang
AU  - Wang JP
AD  - Department of Education and Research, Taichung Veterans General Hospital, 160, 
      Chung Kang Road, Sec. 3, Taiwan, ROC. w1994@vghtc.vghtc.gov.tw
FAU - Chang, Ling-Chu
AU  - Chang LC
FAU - Hsu, Mei-Feng
AU  - Hsu MF
FAU - Huang, Li-Jiau
AU  - Huang LJ
FAU - Kuo, Sheng-Chu
AU  - Kuo SC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (2-benzyloxybenzaldehyde)
RN  - 0 (Benzaldehydes)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Glycerophospholipids)
RN  - 0 (Phosphatidic Acids)
RN  - 0 (phosphatidylethanol)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 3.1.4.4 (Phospholipase D)
RN  - EC 3.6.5.2 (ADP-Ribosylation Factors)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - ADP-Ribosylation Factors/metabolism
MH  - Animals
MH  - Benzaldehydes/*pharmacology
MH  - Calcium/metabolism
MH  - Cell Membrane/drug effects/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Activation/drug effects
MH  - Enzyme Inhibitors/pharmacology
MH  - Glycerophospholipids/metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/antagonists & inhibitors/*pharmacology
MH  - Neutrophils/*drug effects/metabolism
MH  - Phosphatidic Acids/metabolism
MH  - Phospholipase D/antagonists & inhibitors/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - rhoA GTP-Binding Protein/metabolism
EDAT- 2002/10/18 04:00
MHDA- 2002/12/10 04:00
CRDT- 2002/10/18 04:00
PHST- 2002/10/18 04:00 [pubmed]
PHST- 2002/12/10 04:00 [medline]
PHST- 2002/10/18 04:00 [entrez]
AID - S030441650200329X [pii]
AID - 10.1016/s0304-4165(02)00329-x [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2002 Oct 10;1573(1):26-32. doi: 
      10.1016/s0304-4165(02)00329-x.