PMID- 12385578
OWN - NLM
STAT- MEDLINE
DCOM- 20030421
LR  - 20081121
IS  - 0392-9078 (Print)
IS  - 0392-9078 (Linking)
VI  - 21
IP  - 3
DP  - 2002 Sep
TI  - The promoter polymorphism of the matrix metalloproteinase 3 (MMP-3) gene in women 
      with ovarian cancer.
PG  - 357-61
AB  - Matrix metalloproteinases (MMPs) comprise family proteolytic enzymes that degrade 
      extracellular matrix components and therefore play an important role in tumour 
      cell invasion and cancer metastasis. Overexpression of matrix metalloproteinase 3 
      (MMP-3 or stromelysin 1) gene has been demonstrated in various types of cancers 
      and high level of MMP-3 protein in tumour is a poor prognostic factor for 
      patients. The insertion (6A)/deletion (5A) polymorphism (5A/6A polymorphism) 
      located at the promoter of the MMP-3 gene may have functional significance in the 
      regulation of its expression. In the present work the distribution of genotypes 
      and frequencies of alleles of the 5A/6A polymorphism in subjects with ovarian 
      cancer were investigated. Paraffin embedded tumour tissues were obtained from 100 
      women with ovarian cancer. The genotypes of 5A/6A polymorphism were determined by 
      PCR amplification using the allele specific primers. The distribution of the 
      genotypes of the 5A/6A polymorphism in both control and patients did not differ 
      significantly (p > 0.05) from those predicted by the Hardy-Weinberg distribution. 
      Additionally, there were no significant differences (p > 0.05) in genotype 
      distributions and allele frequencies between subgroups assigned to histological 
      stage. The results suggest that the 5A/6A polymorphism of MMP-3 gene may not be 
      linked with appearance and development to ovarian cancer.
FAU - Szyllo, K
AU  - Szyllo K
AD  - Dept. of Surgical Gynaecology, Institute of Polish Mother's Memorial Hospital, 
      Lodz.
FAU - Smolarz, B
AU  - Smolarz B
FAU - Romanowicz-Makowska, H
AU  - Romanowicz-Makowska H
FAU - Niewiadomski, M
AU  - Niewiadomski M
FAU - Kozlowska, E
AU  - Kozlowska E
FAU - Kulig, A
AU  - Kulig A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - J Exp Clin Cancer Res
JT  - Journal of experimental & clinical cancer research : CR
JID - 8308647
RN  - 0 (DNA Primers)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Alleles
MH  - DNA Primers/chemistry
MH  - Female
MH  - Genotype
MH  - Humans
MH  - Loss of Heterozygosity
MH  - Matrix Metalloproteinase 3/*genetics
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Ovarian Neoplasms/*enzymology/genetics/pathology
MH  - Polymerase Chain Reaction
MH  - *Polymorphism, Genetic
MH  - Promoter Regions, Genetic
EDAT- 2002/10/19 04:00
MHDA- 2003/04/22 05:00
CRDT- 2002/10/19 04:00
PHST- 2002/10/19 04:00 [pubmed]
PHST- 2003/04/22 05:00 [medline]
PHST- 2002/10/19 04:00 [entrez]
PST - ppublish
SO  - J Exp Clin Cancer Res. 2002 Sep;21(3):357-61.